Neuronal Cell Death

Methods and Protocols

Laura Lossi, Adalberto Merighi (Herausgeber)

Buch | Softcover

396 Seiten

2016 | Softcover reprint of the original 1st ed. 2015
Humana Press Inc. (Verlag)
978-1-4939-4464-4 (ISBN)

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This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.

Neuronal Cell Death: An Overview of its Different Forms in Central and Peripheral Neurons.- Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy.- Flow Cytometric Analysis of DNA Synthesis and Apoptosis in Central Nervous System Using Fresh Cell Nuclei.- Nuclear Signs of Pre-Neurodegeneration.- Multi-Parametric O2 Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent Probes.- Calcium Imaging in Neuron Cell Death.- Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling through Electron Microscopy Analysis.- Real-Time Visualization of Caspase-3 Activation by Fluorescence Resonance Energy Transfer (FRET).- Design and Cloning of Short Hairpin RNAs (shRNAs) into a Lentiviral Silencing Vector to .- Genomic Analysis Using Affymetrix Standard Microarray GeneChips (169 Format) in Degenerate Murine Retina.- Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis.- High-Throughput Cell Death Assays.- Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death.- In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death.- Laser Microbeam Targeting of Single Nerve Axons in Cell Culture.- Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy.- Targeted Toxicants to Dopaminergic Neuronal Cell Death.- Stem Cells, Neural Progenitors, and Engineered Stem Cells.- Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant Vectors for Gene Transfer and Gene Therapy.- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors for Gene Transfer and Gene Therapy.- Bone Marrow Transplantation for Research and Regenerative Therapies in the Central Nervous System.- Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA).- Generation of Zebrafish Models by CRISPR/Cas9 Genome Editing.- InVivo Assessment of Neuronal Cell Death in Drosophila.- Drosophila Model for Studying Phagocytosis Following Neuronal Cell Death.

Erscheinungsdatum	11.08.2016
Reihe/Serie	Methods in Molecular Biology ; 1254
Zusatzinfo	48 Illustrations, color; 34 Illustrations, black and white; XIV, 396 p. 82 illus., 48 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	calcium concentration • cell viability • Cell volume • DNA synthesis/alterations • dying neurons • epifluorescence and digital holography • manipulate neuronal stem cells and progenitors • mitochondrial function • Neuropathology • RNA silencing
ISBN-10	1-4939-4464-9 / 1493944649
ISBN-13	978-1-4939-4464-4 / 9781493944644
Zustand	Neuware