Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors
Springer International Publishing (Verlag)
978-3-319-45383-5 (ISBN)
The author, Maximilian Oberleitner, describes approaches in which the cells are directly grown on different sensor surfaces (gold-film electrodes, shear wave resonators or dye-doped polymer films). This approach, with the reacting cells in particularly close proximity and contact with the sensor surface, is key to a remarkable sensitivity, opening the way for a variety of new applications. This thesis not only introduces the fundamentals of each approach, but it also describes in great detail the designprinciples and elucidates the boundary conditions of the new sensors.
Maximilian Oberleitner was born in 1983 in Bavaria, Germany and graduated from the University of Regensburg with a Diploma in Chemistry in 2008. Based on this doctoral thesis “Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors” he obtained his PhD in Analytical Chemistry in 2016 in the Institute of Analytical Chemistry, Chemo- and Biosensors at the University of Regensburg under the supervision of Prof. Dr. Joachim Wegener. His research work was focused on the development and improvement of substrate-integrated sensors for the analysis of living cells. These sensors enable the monitoring of cell-substrate interactions and the real-time analysis of assays with adherently grown cells in a non-invasive, label-free and – by combinations of the different types of sensors – even in a multi-parametric manner. Since 2015 he works at a global healthcare company in Austria. As project leader in the quality unit he is responsible for stability studies and quality evaluations of antibiotics.
Introduction.- Objectives.- Methods & Instrumentation.- MQCM: Multiple Cytomechanic Sensing.- QCM-ECIS: Combined Viscoelastic and Dielectric Sensing of Cells.- QCM-OCS: Optochemical Sensing of Temperature and pO2 in the Cell Surface Junction.- Summary.- Appendix.
| Erscheinungsdatum | 03.01.2017 |
|---|---|
| Reihe/Serie | Springer Theses |
| Zusatzinfo | XXVI, 370 p. 138 illus., 122 illus. in color. |
| Verlagsort | Cham |
| Sprache | englisch |
| Maße | 155 x 235 mm |
| Themenwelt | Naturwissenschaften ► Chemie ► Analytische Chemie |
| Schlagworte | Analytical Chemistry • Biological recognition unit • Biophysics • Cell-based assays • Cell Culture • cellular biology (cytology) • Chemistry and Materials Science • Confocal laser scanning microscopy • Cytomechanic sensing • Dynamic cell response • Electric cell-substrate impedance sensing • Fluorescence lifetime imaging • Impedance spectroscopy • Label-free high-content sensing • laboratory medicine • Medical laboratory testing and techniques • Mulitparametric whole-cell biosensing • nanochemistry • nanotechnology • Optical temperature and oxygen imaging • Physics of the Cell • Quartz crystal microbalance |
| ISBN-10 | 3-319-45383-1 / 3319453831 |
| ISBN-13 | 978-3-319-45383-5 / 9783319453835 |
| Zustand | Neuware |
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