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Technical Aspects of Toxicological Immunohistochemistry (eBook)

System Specific Biomarkers

Syed A. Aziz, Rekha Mehta (Herausgeber)

eBook Download: PDF
2015 | 1st ed. 2016
XI, 231 Seiten
Springer New York (Verlag)
978-1-4939-1516-3 (ISBN)

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This authoritative volume examines immunohistochemical methods aimed at investigating the toxicologic pathology of rodent, non-human primate and aquatic animal tissues. Eleven comprehensive chapters provide pathologists and researchers in various sub-disciplines of toxicology with a comprehensive review of the methods and approaches for immunohistochemical staining in various target tissues. It explores the tissue-antigen and antibody-specific problems that may be encountered during the staining procedures and provide potential avenues for resolving various methodological issues. Special attention is paid to the latest enhancement procedures for antigen retrieval and visualization as well as image analysis and antigen quantification. Written by leading researchers in toxicology and pathology, this book is a significant resource for toxicologists and pathologists working with rodents, monkeys and aquatic animal tissues.

Syed A Aziz, Ph.D.

Regulatory Toxicology Research Division, Bureau of Chemical Safety

Food Directorate, Health Products and Food Branch

Health Canada

Adjunct professor, Faculty of Medicine Dept. of Pathology

University of Ottawa

Rekha Mehta, Ph.D.

Regulatory Toxicology Research Division, Bureau of Chemical Safety

Food Directorate, Health Products and Food Branch

Health Canada.


This authoritative volume examines immunohistochemical methods aimed at investigating the toxicologic pathology of rodent, non-human primate and aquatic animal tissues. Eleven comprehensive chapters provide pathologists and researchers in various sub-disciplines of toxicology with a comprehensive review of the methods and approaches for immunohistochemical staining in various target tissues. It explores the tissue-antigen and antibody-specific problems that may be encountered during the staining procedures and provide potential avenues for resolving various methodological issues. Special attention is paid to the latest enhancement procedures for antigen retrieval and visualization as well as image analysis and antigen quantification. Written by leading researchers in toxicology and pathology, this book is a significant resource for toxicologists and pathologists working with rodents, monkeys and aquatic animal tissues.

Syed A Aziz, Ph.D.Regulatory Toxicology Research Division, Bureau of Chemical SafetyFood Directorate, Health Products and Food BranchHealth CanadaAdjunct professor, Faculty of Medicine Dept. of PathologyUniversity of OttawaRekha Mehta, Ph.D.Regulatory Toxicology Research Division, Bureau of Chemical SafetyFood Directorate, Health Products and Food BranchHealth Canada.

1.Immunohistochemistry – Overview, Its Potential and Challenges.- 2.Tissue Micro Array (TMA) Technology and its Potential Applications in Toxicology and Toxicological Immunohistochemistry.- 3.Importance of Fluorescent In Situ Hybridization (FISH) in Rodent Tumors.- 4.Quantitation of Immunohistochemistry by Image Analysis Technique.- 5.Immunohistochemistry in the Study of Cancer Biomarkers for Oncology Drug Development.- 6.Male Reproductive Toxicology and the Role of Immunohistochemistry.- 7. Immunohistochemistry and Female Reproductive Toxicology: The Ovary and Mammary Gland.- 8.Immunohistochemistry on Rodent Circulatory System: Its Possible Use in Investigating Hypertension.- 9.Role of Lymphoid/Mucosa-Associated Lymphoid Tissue Markers in Toxicological Immunohistochemistry. 10.Bone Markers and Immunohistochemistry Changes in Toxic Environments.- 11. Biomarkers of Skeletal Muscle Injury in the Rat.

Erscheint lt. Verlag 23.12.2015
Zusatzinfo XI, 231 p. 53 illus., 3 illus. in color.
Verlagsort New York
Sprache englisch
Themenwelt Medizin / Pharmazie Allgemeines / Lexika
Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
Naturwissenschaften Biologie Zellbiologie
Technik
Schlagworte Biological Microscopy • Cell Biology • Diagnostic Pathology • Immunocytochemistry • tissue biology • Toxicologic pathology • tumor diagnosis
ISBN-10 1-4939-1516-9 / 1493915169
ISBN-13 978-1-4939-1516-3 / 9781493915163
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