Chikungunya Virus
Humana Press Inc. (Verlag)
978-1-4939-3616-8 (ISBN)
volume covers multidisciplinary approaches on various aspects of Chikungunya
Virus (CHIKV) research that was brought together from leading laboratories
across the globe. Topics covered include techniques in clinical and diagnostic
virology, basic protocols in cell and virus culture, bioinformatics, and
proteomics approaches in cellular response studies. Chikungunya Virus:
Methods and Protocols also discusses methods in immunology and animal model
studies, as well as different strategies of antivirals and vaccine development
for therapeutics against CHIKV infection. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary material and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.
Thorough and practical, Chikungunya Virus: Methods and Protocols is a
useful and comprehensive resource for CHIKV researchers and anyone interested
in learning more about the Chikungunya Virus.
Evolution and Epidemiology
of Chikungunya Virus.- Detection
and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay.- Advanced Genetic Methodologies
in Tracking Evolution and Spread of Chikungunya Virus.- Synthetic
Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with
and without Neurological Complications.- Expression and Purification of E2 Glycoprotein from Insect
Cells (Sf9) for Use in Serology.- Diagnostic Methods for CHIKV Based on Serological Tools.- Utilization and Assessment of Throat Swab and Urine Specimens
for Diagnosis of Chikungunya Virus Infection.- Propagation
of Chikungunya Virus Using Mosquito Cells.- Infectious
Viral Quantification of Chikungunya Virus - Virus Plaque Assay.- Molecular
Epidemiology of Chikungunya Virus by Sequencing.- Chikungunya Virus Infection of Aedes Mosquitoes.- Analysis
of CHIKV in Mosquitoes Infected via Artificial Blood Meal.- Chikungunya
Virus Growth and Fluorescent Labeling: Detection of Chikungunya Virus by Immunofluorescence
Assay.- Virus Isolation and Preparation of Sucrose-Banded
Chikungunya Virus Samples for Transmission Electron Microscopy.- Viral-Host
Protein Interaction Studies Using Yeast Two-Hybrid Screening Method.- Application of GelC-MS/MS to Proteomic Profiling of Chikungunya Virus Infection:
Preparation of Peptides for Analysis.- Bioinformatics
Based Approaches to Study Virus-Host Interactions During Chikungunya Virus Infection.- T-Cell Epitope Prediction of
Chikungunya Virus.- Mouse
Models of Chikungunya Virus.- Generation
of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY
Hybridoma Cloning Kit.- Immunohistochemical
Detection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-Embedded
Tissues.- Antiviral Strategies against Chikungunya Virus.- A Real Time Cell Analyzing Assay for Identification
of Novel Antiviral Compounds against Chikungunya Virus.- Using Bi-Cistronic Baculovirus Expression Vector System to Screen the Compounds
that Interfere the Infection of Chikungunya Virus.- Neutralization
Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test.- Reverse
Genetics Approaches for Chikungunya Virus.- Production
of Chikungunya Virus-Like Particles and Subunit Vaccines in Insect Cells.- Protocols for Developing Novel Chikungunya Virus DNA
Vaccines.
| Erscheinungsdatum | 20.04.2016 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1426 |
| Zusatzinfo | 41 Illustrations, color; 11 Illustrations, black and white; XIV, 335 p. 52 illus., 41 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
| Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | alphavirus genus • cell and virus culture • cellular response • Chikungunya Virus (CHIKV) • clinical and diagnostic virology • Togaviridae • vaccine development for therapeutics |
| ISBN-10 | 1-4939-3616-6 / 1493936166 |
| ISBN-13 | 978-1-4939-3616-8 / 9781493936168 |
| Zustand | Neuware |
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