Optogenetics

Methods and Protocols

Buch | Hardcover

408 Seiten

2016 | 1st ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-3510-9 (ISBN)

Artikel merken

This volume provides researchers with up-to-date protocols and takes aclose look at current research and promising applications.

This volume provides researchers with up-to-date protocols and takes a
close look at current research and promising applications. Optogenetics:
Methods and Protocols contains a collection of recently developed technical
protocols on optogenetic applications in neuroscience, brain mapping, treatment
of neurological disorders, and restoration of visual function. Several
introductory and discussion chapters offer a wide overview about sources and
diversity of optogenetic tools, design strategies, and potential applications
in other fields like plant research. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.

Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers
an interdisciplinary view of optogenetic applications and its potential to
develop as a valuable tool for basic research, as well as biomedical and
biotechnological applications.

Optogenetics:
Basic Concepts and Their Development.- Natural
Resources for Optogenetic Tools.- Algal Photobiology: A Rich Source of
Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics.- Reversible Photoregulation of gene
Expression and Translation.- Controlling Protein Activity and
Degradation Using Blue-Light.- Photo-Control of Protein Function Using
Photoactive Yellow Protein.- A Fluorometric Activity Assay for
Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators.- Optogenetic Control of Pancreatic Islets.- Optogenetics in Plants: Red/Far-Red Light
Control of Gene Expression.- Perfect Channelrhodopsins: A User Guide.- Optogenetics in Drosophila Neuroscience.- Optogenetic Control of Mammalian Ion
Channels with Chemical Photoswitches.- Optogenetic Modulation of Locomotor
Activity on Free Behaving Rats.- Combines Optogenetic and Chemogenetic
Control of Neurons.- Intracranial Injection ofan Optogenetics
Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in
Rat Brain Cortex.- An Optomized Calcium-Phosphate Transfection
Method for Characterizing Genetically Encoded Tools in Primary Neurons.- Optogenetic Approaches for Mesoscopic Brain
Mapping.- Optogenetic Tools for Confined Stimulation
in Deep Brain Structures.- Remote Patterning of Transgene Expression
Using Near Infrared-Responsive Plasmonic Hydrogels.- Optogenetic Light Crafting Tools for the
Control of Cardiac Arrhythmias.- Inscribing Optocal Excitability to
Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary
Cardiac Fibroblasts.- Optogenetic Engineering of Atrial
Cardiomyocytes.- A Multi-Channel Recording System with
Optical Stimulation for Closed-Loop Optogenetic Experiments.- Optogenetic Control of Fibroblast Growth
Factor Receptor Signaling.- Protein Inactivation by Optogenetic
Trapping in Living Cells.- Optogenetic Manipulation of Selective
Neural Activity in Free-Moving Drosophila
Adults.- Guidelines for Photoreceptor Engineering.

Erscheinungsdatum	08.10.2016
Reihe/Serie	Methods in Molecular Biology ; 1408
Zusatzinfo	98 Illustrations, color; 13 Illustrations, black and white; XIII, 408 p. 111 illus., 98 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 1. Studienabschnitt (Vorklinik) ► Anatomie / Neuroanatomie
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	algal sensory photoreceptors • cellular signaling pathways • channelrhodopsins • gene expression • Genome sequencing • neural spiking with light • photoswitchable proteins • protein activity • protein localization • transcriptome sequencing
ISBN-10	1-4939-3510-0 / 1493935100
ISBN-13	978-1-4939-3510-9 / 9781493935109
Zustand	Neuware