Visualization of Receptors In Situ
Applications of Radioligand Binding
Seiten
2000
Crc Press Inc (Verlag)
978-0-8493-0042-4 (ISBN)
Crc Press Inc (Verlag)
978-0-8493-0042-4 (ISBN)
Radioligand binding in situ is the most sensitive method for detecting available receptors. Based on the authors' experience in the field, this title details these procedures and provides step-by-step methods for visualizing receptors via radioligand binding. It covers various practical procedures, including radioligand and tissue preparations.
Radioligand binding in situ is the most sensitive method for detecting available receptors; the success and reliability of this approach are determined by a number of technical requirements and constraints. Based on the authors' fifteen years of experience in the field, Visualization of Receptors In Situ: Applications of Radioligand Binding explicitly details these procedures - often not encountered in the original research papers - helping the reader overcome the challenges associated with this powerful technique.
Radioligand binding in situ is the most sensitive method for detecting available receptors; the success and reliability of this approach are determined by a number of technical requirements and constraints. Based on the authors' fifteen years of experience in the field, Visualization of Receptors In Situ: Applications of Radioligand Binding explicitly details these procedures - often not encountered in the original research papers - helping the reader overcome the challenges associated with this powerful technique.
Emmanuel Moyse, Slavica M Krantic
LIGHT MICROSCOPY. Ligand Labeling with Radioisotopes. Tissue Preparation for Light Microscopy. Radioligand Binding Assay on Frozen Tissue Sections. Film (Dry) Radioautography. Liquid Emulsion (Wet) Radioautography. Quantification of Radioautographic Labeling. ELECTRON MICROSCOPY. Choice of Radioligand. Tissue Preparation. Radioligand Binding Assay on Thick Tissue Slices. Embedding and Ultrathin Sectioning. Radioautography. Coloration and Contrasting of Ultrathin Sections. Analysis and Quantification of Radioautographic Labeling.
| Erscheint lt. Verlag | 30.11.2000 |
|---|---|
| Reihe/Serie | Methods in Visualization |
| Zusatzinfo | 7 Tables, black and white; 19 Halftones, black and white |
| Verlagsort | Bosa Roca |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Gewicht | 586 g |
| Themenwelt | Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Naturwissenschaften ► Physik / Astronomie | |
| ISBN-10 | 0-8493-0042-8 / 0849300428 |
| ISBN-13 | 978-0-8493-0042-4 / 9780849300424 |
| Zustand | Neuware |
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