Environmental Responses in Plants

Methods and Protocols

Buch | Hardcover

387 Seiten

2016 | 1st ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-3354-9 (ISBN)

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This volume describes different up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Environmental Responses in Plants: Methods and Protocols is divided into four sections: Tropisms, Photoperiodism and Circadian Rhythms, Abiotic Stress Responses, and Plant-Pathogen Interactions. The chapters in these sections include detailed protocols to investigate some of the many key biological processes underlying plant environmental responses, mostly in the model organism Arabidopsis thaliana, but also in Physcomitrella patens and in different crop species such as rice, potato, barley, or tomato. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Environmental Responses in Plants: Methods and Protocols, is a great resource for plant physiologists, biochemists, and cell and molecular scientists interested in this exciting and fast-growing research topic.

Paula Duque Plant Molecular Biology group Instituto Gulbenkian de Ciência Rua da Quinta Grande, 6 2780-156 Oeiras Portugal Phone: +351 214464630 Fax: +351 214407970 Email: duquep@igc.gulbenkian.pt

Hydrotropism: Analysis of the Root Response to a Moisture Gradient.- Assessing Gravitropic Responses in Arabidopsis.- Physiological Analysis of Phototropic Responses in Arabidopsis.- Automatic Chloroplast Movement Analysis.- Microscopic and Biochemical Visualization of Auxins in Plant Tissues.- Immunolocalization of PIN and ABCB Transporters in Plants.- Analysis of Circadian Leaf Movements.- Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes.- A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are Temporally Restricted by the Circadian Clock.- Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hybrid Screens Using a Transcription Factor ORFeome.- Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes.- Assessing the Impact of Photosynthetic Sugars on the Arabidopsis Circadian Clock.- Assessing Protein Stability Under Different Light and Circadian Conditions.- Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought.- Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana.- Assessing Tolerance to Heavy-Metal Stress in

Arabidopsis Thaliana Seedlings.- Assessing Drought Responses Using Thermal Infrared Imaging.- Generating Targeted Gene Knockout Lines in Physcomitrella Patens to Study Evolution of Stress-Responsive Mechanisms.- Screening Stress Tolerance Traits in Arabidopsis Cell Cultures.- Using Arabidopsis Protoplasts to Study Cellular Responses to Environmental Stress.- Construction of Artificial miRNAs to Prevent Drought Stress in Solanum Tuberosum.- Virus-Induced Gene Silencing for Gene Function Studies in Barley.- Methods for Long-Term Stable Storage of Colletotrichum Species.- Plant Inoculation With the Fungal Leaf Pathogen Colletotrichum higginsianum.- Tracing Plant Defense Responses in Roots Upon MAMP/DAMP Treatment.- Analysis of the Immunity-Related Oxidative Bursts by a Luminol-Based Assay.- Quantitative Analysis of Microbe- Associated-Molecular-Pattern (MAMP)-Induced Ca2+ Transients in Plants.- Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR.- Determining Nucleosome Position at Individual Loci after Biotic Stress Using MNase-qPCR.- Phosphoprotein Enrichment Combined with Phosphopeptide Enrichment to Identify Putative Phosphoproteins During Defense Response in Arabidopsis thaliana.

Erscheinungsdatum	31.12.2015
Reihe/Serie	Methods in Molecular Biology ; 1398
Zusatzinfo	58 Illustrations, color; 21 Illustrations, black and white; XIV, 387 p. 79 illus., 58 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
ISBN-10	1-4939-3354-X / 149393354X
ISBN-13	978-1-4939-3354-9 / 9781493933549
Zustand	Neuware