Lipid Signaling Protocols
Humana Press Inc. (Verlag)
978-1-4939-3169-9 (ISBN)
Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format.- Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass Spectrometry.- Fluorescent Assays for Ceramide Synthase Activity.- Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II Alpha.- Measurement of Long Chain Fatty Acyl-CoA synthetase Activity.- Qualitative and Quantitative in vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specificity.- Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity.- Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microdomains.- Fluorescence Recovery after Photobleaching Analysis of the Diffusional Mobility of Plasma Membrane Proteins: HER3 Mobility in Breast Cancer Cell Membranes.- Isolation and Analysis of Detergent-Resistant Membrane Fractions.- Detection of Isolated Mitochondria-Associated ER Membranes using the Sigma-1 Receptor.- Using Surface Plasmon Resonance to Quantitatively Assess Lipid-Protein Interactions.- Analyzing Protein-phosphoinositide Interactions with Liposome Flotation Assays.- High-Throughput Flurometric Assay for Membrane-Protein Interaction.- Guidelines for the use of Protein Domains in Acidic Phospholipid Imaging.- Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]serine.- Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells.- Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions.- Single-molecule Imaging of Signal Transduction via GPI-anchored Receptors.- Measuring Phosphatidylinositol Generation on Biological Membranes.- Assay for CDP-diacylglycerol Generation by CDS in Membrane Fractions.
Reihe/Serie | Methods in Molecular Biology ; 1376 |
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Zusatzinfo | XI, 257 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Naturwissenschaften ► Chemie ► Organische Chemie | |
Schlagworte | Cell function • Ceramides • cholesterol-rich lipid rafts • detergent-resistant membrane • lipid signaling • membrane environment • phosphoinositides • receptor-initiated signaling cascades • sphingomyelin |
ISBN-10 | 1-4939-3169-5 / 1493931695 |
ISBN-13 | 978-1-4939-3169-9 / 9781493931699 |
Zustand | Neuware |
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