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Single Cell Protein Analysis

Methods and Protocols

Anup K. Singh, Aarthi Chandrasekaran (Herausgeber)

Buch | Hardcover

254 Seiten

2015 | 1st ed. 2015
Humana Press Inc. (Verlag)
978-1-4939-2986-3 (ISBN)

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This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.

Anup Singh Mailstop 9291 Sandia National Laboratories 7011 East Ave Livermore, CA aksingh@sandia.gov

Single-Cell Western Blotting.- A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. Coli Bacteria.- Enzyme-Linked ImmunoSpot (ELISpot) for Single Cell Analysis.- Photocleavable DNA Barcoding Antibodies for Multiplexed Protein Analysis in Single Cells.- Genome-Wide Analysis of Protein and mRNA Copy Numbers in Single Escherichia Coli Cells with Single-Molecule Sensitivity.- Microfluidic Flow Cytometry for Single Cell Protein Analysis.- Microfluidic Image Cytometry for Single Cell Phenotyping of Human Pluripotent Stem Cells.- Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry.- Multiplex Peptide-MHC Tetramer Staining with Mass Cytometry.- Imaging and Mapping of Tissue Constituents at the Single Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry.- SPLIFF: A Single Cell Method to Map Protein-Protein Interactions in Time and Space.- Microfluidic Proximity Ligation Assay for Profiling Signaling Networks with Single-Cell Resolution.- Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells.- Microfluidics Enabled Enzyme Activity Measurement in Single Cells.- Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells.- Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells.

Erscheint lt. Verlag	7.11.2015
Reihe/Serie	Methods in Molecular Biology ; 1346
Zusatzinfo	XI, 254 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	cell signaling • flow cytometry • gel electrophoresis • Heterogeneous • immunoassays • Mass Spectrometry • Microfluids • proteins • Proteome • Translocation
ISBN-10	1-4939-2986-0 / 1493929860
ISBN-13	978-1-4939-2986-3 / 9781493929863
Zustand	Neuware