Advanced Photon Counting (eBook)

Applications, Methods, Instrumentation

Peter Kapusta, Michael Wahl, Rainer Erdmann (Herausgeber)

eBook Download: PDF

2015 | 2015
XII, 370 Seiten
Springer International Publishing (Verlag)
978-3-319-15636-1 (ISBN)

Infos

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a 'fingerprint' for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

Inhalt

Modern TCSPC electronics - Principles and Acquisition ModesMichael Wahl   Single-photon counting detectors for the visible range between 300 nm and 1000 nmAndreas Bülter  Single-photon detectors for infrared wavelengths in the range 1 to 1.7 μmGerald S. Buller and Robert J. Collins Modern pulsed diode laser sources for time-correlated photon countingKristian Lauritsen, Sina Riecke#, Andreas Bülter, Thomas Schönau Advanced FCS: an Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual Focus FCSThomas Dertinger and Steffen Rüttinger Lifetime-weighted FCS and 2D FLCS: Advanced application of time-tagged TCSPCKunihiko Ishii, Takuhiro Otosu, and Tahei Tahara MFD-PIE and PIE-FI: Ways to extract more information with TCSPCAnders Barth, Lena Voith von Voithenberg, Don C. Lamb Photon Antibunching in Single Molecule Fluorescence SpectroscopyKristin S. Grußmayer and Dirk-Peter Herten FLIM Strategies for Intracellular Sensing: Fluorescence Lifetime Imaging as a Tool to Quantify Analytes of InterestMaria J. Ruedas-Rama, Jose M. Alvarez-Pez, Luis Crovetto, Jose M. Paredes, Angel Orte Multiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and TissueRafal Fudala, Ryan M. Rich, Joe Kimball, Ignacy Gryczynski, Sangram Raut,  Julian Borejdo, Dorota L. Stankowska, Raghu R. Krishnamoorthy, Karol Gryczynski, Badri P. Maliwal, Zygmunt Gryczynski Pattern based linear un-mixing for efficient and reliable analysis of multi-component TCSPC-dataIngo Gregor and Matthias Patting Metal-Induced Energy TransferNarain Karedla, Daja Ruhlandt, Anna M. Chizhik, Jörg Enderlein, Alexey I. Chizhik The importance of photon arrival times in STED microscopy Giuseppe Vicidomini Single color centers in diamond as single photon sources and quantum sensorsBoris Naydenov and Fedor Jelezko Photon counting and timing in quantum optics experimentsAndreas Ahlrichs, Benjamin Sprenger, Oliver Benson Photon counting in diffuse optical imagingDirk Grosenick

Produkt-Details

• Erscheint lt. Verlag: 23.4.2015
• Reihe/Serie: Springer Series on Fluorescence
• Reihe/Serie: Springer Series on Fluorescence
• Zusatzinfo: XII, 370 p. 159 illus., 103 illus. in color.
• Verlagsort: Cham
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie
• Themenwelt: Naturwissenschaften ► Chemie ► Physikalische Chemie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie
• Themenwelt: Technik
• Schlagworte: Antibunchng • Coincidence correlation • Diamond defect centers • FCS • FLIM • Fluorescence Correlation Spectroscopy • Fluorescence lifetime • FRET • optical tomography • Single molecule spectroscopy • Single photon detectors • TCSPC • Time-correlated single photon counting • Time interval analysis
• ISBN-10: 3-319-15636-5 / 3319156365
• ISBN-13: 978-3-319-15636-1 / 9783319156361