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Electroporation Methods in Neuroscience

Tetsuichiro Saito (Herausgeber)

Buch | Hardcover

199 Seiten

2015
Humana Press Inc. (Verlag)
978-1-4939-2458-5 (ISBN)

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This volume describes the methods of both in vivo and in vitro electroporation using ferrets, rats, mice, chickens, and zebrafish. Recent advances of experiments using the tetracycline-regulated gene expression and Tol2 transposon systems are also included. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Practical and authoritative, Electroporation Methods in Neuroscience serves to aid scientists in the further study into this crucially important way to study cells.

In utero Electroporation of the Mouse Embryo.- Exo utero Electroporation of the Mouse Embryo.- In Vivo Electroporation of the Postnatal Rodent Forebrain.- Electroporation in the Rodent Retina in vivo and in vitro.- Electroporation in the Developing Mouse Olfactory Bulb.- Cell Migration Analysis of Cortical Interneurons after Electroporation.- Characterization of Newborn Interneurons In The Mouse Olfactory Bulb Using Postnatal Electroporation.- Genetic Manipulation Of Gyrencephalic Carnivores using in utero electroporation.- Electroporation for the Chick Embryonic CNS.- Targeting the Zebrafish Nervous System using In Vivo Electroporation.- Gene Transfer into Cultured Mammalian Embryos by Electroporation.- Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Cultures.- Electroporation of Dissociated Hippocampal Neurons.- Tol2-Mediated Transgenesis And Its Application To Electroporation.- Control of Gene Expression In Neurons by the use of in vivo Electroporation and the Tetracycline System.

Reihe/Serie	Neuromethods ; 102
Zusatzinfo	58 Illustrations, color; 13 Illustrations, black and white; XII, 199 p. 71 illus., 58 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Neurologie
	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	calcium sensors • cellular morphologies • designer receptors • electric pulse • Fluorescent proteins • Gene function • in vitro electroporation • in vivo electroporation • light-gated channel proteins
ISBN-10	1-4939-2458-3 / 1493924583
ISBN-13	978-1-4939-2458-5 / 9781493924585
Zustand	Neuware