Affinity Chromatography
Humana Press Inc. (Verlag)
978-1-4939-2446-2 (ISBN)
Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.
Affinity Chromatography: A Historical.- Protein Purification by Aminosquarylium Cyanine-Affinity Chromatography.- One Step Purification of Phosphinothricin Acetyltransferase using Reactive Dye Affinity Chromatography.- Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes.- Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography using a Synthetic Aromatic Ligand.- Cell Affinity Separations on Microfluidic Devices.- Aptamer-modified :Magnetic Beads in Affinity Separation of Proteins.- The Strep-tag System for One-step Purification and High Affinity Detection or Capturing of Proteins.- Robotic High-throughput Purification of Affinity-tagged Recombinant Proteins.- Macroporous Silica Particles Derivatized for Enhanced Lectin Affinity Enrichment of Glycoproteins.- Immobilized Magnetic Beads based Multi-Target Affinity Selection Coupled with HPLC-MS for Screening Active Compounds from Traditional Chinese Medicine and Natural Products.- Mixed-bed Affinity Chromatography: Principles And Methods.- Preparation and characterization of fluorophenylboronic acid-functionalized Affinity Monolithic Columns for the Selective Enrichment of Cis-diol-containing Biomolecules.- Introduction to Macroporous Cryogels.- Cryogels with Affinity Ligands as Tools in Protein Purification.- Direct Capture of His6-tagged Proteins Using Megaporous Cryogels Developed for Metal-ion Affinity Chromatography.- Ni(II) Chelated IDA Functionalized Poly(HEMA-GMA) Cryogels for Urease Adsorption.- A Novel Chromatographic Media: Histidine Containing Composite Cryogels for HIgG Separation from Human Serum.- Molecularly Imprinted Cryogels for Human Serum Albumin Depletion.- Interpenetrating Polymer Network Composite Cryogels with Tailored Porous Morphology and Sorption Properties.- Analysis of Drug-Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin.- Accurateprotein-peptide Titration Experiments by Nuclear Magnetic Resonance using Low-volume Samples.- Characterization of the Binding Strengths between Boronic Acids and Cis-Diol-containing Biomolecules by Affinity Capillary Electrophoresis.- Determination of the Kinetic Rate Constant of Cyclodextrin Supramolecular Systems by High Performance Affinity Chromatography.- Molecular Modeling of the Affinity Chromatography of Monoclonal Antibodies.
| Reihe/Serie | Methods in Molecular Biology ; 1286 |
|---|---|
| Zusatzinfo | 37 Illustrations, color; 41 Illustrations, black and white; XIII, 341 p. 78 illus., 37 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Sachbuch/Ratgeber ► Natur / Technik ► Naturführer |
| Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Chemie ► Analytische Chemie | |
| Schlagworte | binding constants • cryogelation process • dye affinity chromatography • immobilized metal-chelated affinity chromatography • kinetic rate • macroporous monoliths • mass transfer • polymeric network • quantitative isolation of biomolecules |
| ISBN-10 | 1-4939-2446-X / 149392446X |
| ISBN-13 | 978-1-4939-2446-2 / 9781493924462 |
| Zustand | Neuware |
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