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Advanced Fluorescence Microscopy

Methods and Protocols

Peter J. Verveer (Herausgeber)

Buch | Hardcover

294 Seiten

2014
Humana Press Inc. (Verlag)
978-1-4939-2079-2 (ISBN)

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This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications.- Two-Photon Excitation Microscopy And Its Applications In Neuroscience.- Live Spheroid Formation Recorded with Light Sheet-based Fluorescence Microscopy.- Fluorescence Microscopy Based RNA Interference Screening.- Fluorescence Resonance Energy Transfer Microscopy (FRET).- Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy.- Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation.- Fluorescence Correlation Spectroscopy.- Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules.- Practical Structured-Illumination Microscopy.- 4Pi Microscopy of the Nuclear Pore Complex.- Application Of STED Microscopy To Cell Biology Questions.- Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes.- Direct Stochastic Optical Reconstruction Microscopy (dSTORM).- Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells.

Reihe/Serie	Methods in Molecular Biology ; 1251
Zusatzinfo	65 Illustrations, color; 21 Illustrations, black and white; XI, 294 p. 86 illus., 65 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizinische Fachgebiete ► Radiologie / Bildgebende Verfahren ► Radiologie
	Naturwissenschaften ► Biologie
	Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
Schlagworte	Biological Systems • Cell Biology • fluorescence light microscope • Fluorescence Microscopy • molecular • Neurobiology
ISBN-10	1-4939-2079-0 / 1493920790
ISBN-13	978-1-4939-2079-2 / 9781493920792
Zustand	Neuware