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RNA-RNA Interactions

Methods and Protocols

Frank J. Schmidt (Herausgeber)

Buch | Hardcover

219 Seiten

2014
Humana Press Inc. (Verlag)
978-1-4939-1895-9 (ISBN)

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In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Thorough and intuitive, RNA-RNA Interactions: Methods and Protocols guides scientists investigating biological systems and studying RNA.

Identifying RNA Recombination Events and Non-covalent RNA-RNA Interactions with the Molecular Colony Technique.- RNA-directed Recombination of RNA in Vitro.- RNA-RNA Selex.- Identification of Antisense RNA S0tem-loops that Inhibit RNA-protein Interactions using a Bacterial Reporter System.- Trans-activation of Large Ribozymes.- Native Purification and Labeling of RNA for Single Molecule Fluorescence Studies.- Single Molecule Studies of RNA-RNA Interactions.- Modification Interference Analysis of the Ribosome.- Assessing Intermolecular RNA:RNA Interactions Within a Ribonucleoprotein Complex Using Heavy Metal Cleavage Mapping.- Monitoring RNA-RNA Interactions With Electrophoretic Mobility Shift Assays: Analysis Of Trna Binding To The T Box Riboswitch Antiterminator RNA.- Electrophoretic Mobility Shift Assay of RNA-RNA Complexes.- Structural Studies of a Double-stranded RNA from Trypanosome RNA Editing by Small Angle X-ray Scattering.- Fusion RNAs in Crystallographic Studies of Double-stranded RNA from Trypanosome RNA Editing.

Reihe/Serie	Methods in Molecular Biology ; 1240
Zusatzinfo	6 Illustrations, color; 31 Illustrations, black and white; X, 219 p. 37 illus., 6 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	bacterial cells • catalyze chemical reactions • gene expression • peptide bond • regulatory RNAs • RNA • RNA-RNA interactions • small RNAs • synthesis
ISBN-10	1-4939-1895-8 / 1493918958
ISBN-13	978-1-4939-1895-9 / 9781493918959
Zustand	Neuware