Exocytosis and Endocytosis
Humana Press Inc. (Verlag)
978-1-4939-0943-8 (ISBN)
Highly practical and clearly written, Exocytosis and Endocytosis, Second Edition will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms.
Pharmacological Inhibitors of Exocytosis and Endocytosis: Novel Bullets for Old Targets.- Systematic Analysis of Endocytosis by Cellular Perturbations.- Real Time Detection of SNARE Complexes with FRET Using the Tetracysteine System.- Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides.- Secretion of Circular Proteins Using Sortase.- Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-Epithelial Cells by OptiPrepTM Density Gradient Ultracentrifugation.- Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis.- Probabilistic Density Maps to Study the Spatial Organization of Endocytosis.- Use of Kaede and Kikume Green-Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors.- Halo Tag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells.- SNAP-Tag to Monitor Trafficking of Membrane Proteins in Polarized Epithelial Cells.- FLAsH-PALM: Super-Resolution Pointillist Imaging with FLAsH-tetracysteine Labeling.- Analysis of Protein Dynamics with Tandem Fluorescent Protein Timers.- Synchronization of Secretory Cargo Trafficking in Populations of Cells.- Use of Transmembrane FRET to Investigate the Internalization of Glycosylated Proteins.- A Method to Rapidly Induce Organelle-specific Molecular Activities and Membrane Tethering.- A Novel Pair of Split Venus Fragments to Detect Protein-Protein Interactions by In Vitro and In Vivo Biomolecular Fluorescence Complementation Assays.- Real Time Investigation of Plasma Membrane Deformation and Fusion Pore Expansion Using Polarized Total Internal Reflection Fluorescence Microscopy.- Nanocones to Study Initial Steps of Endocytosis.- A Novel Permeabilization Protocol to Obtain Intracellular 3D Immunolabeling for Electron Tomography.- VIS2FIX: Rapid Chemical Fixation of Vitreous Sections for Immuno-Electron Microscopy.- Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using theEndomembrane Trafficking Network.- Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in C. elegans.- Visualization of Clathrin-Mediated Endocytosis in Live Drosophila Egg Chambers.- Novel Extraction Protocol to Probe the Role of Cholesterol in Synaptic Vesicle Recycling.- Microfluidic Devices for Imaging Trafficking Events In Vivo Using Genetic Model Organisms.- The “In Situ” Proximity Ligation Assay to Probe Protein-Protein Interactions in Intact Tissues.- Probing the Role of the Actin Cytoskeleton During Regulated Exocytosis by Intravital Microscopy.- Measurement of Dynamic F-actin Changes During Exocytosis.
| Reihe/Serie | Methods in Molecular Biology ; 1174 |
|---|---|
| Zusatzinfo | 55 Illustrations, color; 38 Illustrations, black and white; XIII, 435 p. 93 illus., 55 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | biochemical assays • Cultured cells • endocytosis • Exocytosis • live cell imaging • vesicular trafficking |
| ISBN-10 | 1-4939-0943-6 / 1493909436 |
| ISBN-13 | 978-1-4939-0943-8 / 9781493909438 |
| Zustand | Neuware |
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