Dengue
Humana Press Inc. (Verlag)
978-1-4939-0347-4 (ISBN)
Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.
Dengue Virus Growth, Purification and Fluorescent Labeling.- Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique.- Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique.- Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy.- Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion.- Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated
Neutralization and Enhancement of Dengue Virus Infection.- Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging.- Development and Application of Dengue Virus Reverse Genetic Systems.- DENV4 Renilla Luciferase Reporter Replicons.- Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo
Recombination.- Identification of Dengue-Specific Human Antibody Fragments Using Phage Display.- Next Generation Whole Genome Sequencing of Dengue Virus.- MPGAfold in Dengue Secondary Structure Prediction.- Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE.- Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5.- Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry.- Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore.- Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation.- Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import.- Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening.- Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors.- Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase.- Animal Models in Dengue.- Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model.
Reihe/Serie | Methods in Molecular Biology ; 1138 |
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Zusatzinfo | 49 Illustrations, color; 33 Illustrations, black and white; XIII, 403 p. 82 illus., 49 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin |
Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin | |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-4939-0347-0 / 1493903470 |
ISBN-13 | 978-1-4939-0347-4 / 9781493903474 |
Zustand | Neuware |
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