Homing Endonucleases
Humana Press Inc. (Verlag)
978-1-62703-967-3 (ISBN)
Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.
Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers.- Bioinformatic Identification of Homing Endonucleases and Their Target Sites.- PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes.- Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein.- Mapping Free-Standing Homing Endonuclease Promoters Using 5’RLM-RACE.- PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis.- A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleases.- Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selection.- A Yeast-Based Recombination Assay for Homing Endonuclease Activity.- Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile.- Quantifying the Information Content of Homing Endonuclease Target Sites by Single Base Pair Profiling.- Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries.- Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display.- Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes.- Bioinformatics Identification of Coevolving Residues.- Identification and Analysis of Genomic Homing Endonuclease Target Sites.- Redesigning the Specificity of Protein-DNA Interactions with Rosetta.
Reihe/Serie | Methods in Molecular Biology ; 1123 |
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Zusatzinfo | 39 Illustrations, color; 21 Illustrations, black and white; X, 284 p. 60 illus., 39 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Technik ► Umwelttechnik / Biotechnologie | |
ISBN-10 | 1-62703-967-8 / 1627039678 |
ISBN-13 | 978-1-62703-967-3 / 9781627039673 |
Zustand | Neuware |
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