FISH Technology

Bernd W. Rautenstrauß, Thomas Liehr (Herausgeber)

Buch

X, 494 Seiten

2002 | 1., Ed.
Springer Berlin (Verlag)
978-3-540-67276-0 (ISBN)

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Fluorescence in situ hybridization (FISH) is a powerful method to directly visualise the localisation of genomic alterations in the nucleus. The technique has been adapted to a wide range of applications in both medicine, especially diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis-related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH); FISH on insect cells and yeast; multi-color FISH applications (spectral karyotyping analysis, reverse FISH and FICTION); FISH-related techniques, such as PRINS and microarrays.

From the Contents:
- The Standard FISH Procedure - an Introduction;
- FISH on Native, Human Tissues;
- FISH on Archival, Human Tissues;
- Special FISH Techniques (Fiber-FISH, PNA-FISH, CO-FISH, NU-FISH);
- FISH in Zoology and Virology;
- Multicolor FISH Applications;
- Not FISH but Almost;
- Appendix: Internet Sites Related to Cytogenetics and Molecular Cytogenetics

Reihe/Serie	Springer Lab Manual
Sprache	englisch
Maße	193 x 242 mm
Gewicht	1152 g
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Biologische Medizin • Biologische Medizin / Biomedizin • cytogenetics • DNA • genomic alterations • HC/Biologie/Genetik, Gentechnik • HC/Biologie/Mikrobiologie • In situ Hybridization • karyotyping
ISBN-10	3-540-67276-1 / 3540672761
ISBN-13	978-3-540-67276-0 / 9783540672760
Zustand	Neuware