The Molecular Biology of Bacterial Virus Systems

Buch | Softcover

VII, 90 Seiten

2011 | 1. Softcover reprint of the original 1st ed. 1988
Springer Berlin (Verlag)
978-3-642-73117-4 (ISBN)

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The fact that none of the known DNA polymerases is able to initiate DNA chains but only to elongate from a free 3' -OH group raises the problem of how replication is initiated, both at the replication origin and on Okazaki frag ments. It was first shown by A. KORNBERG et al. that a general mechanism to initiate replication is through the formation of an RNA primer catalyzed by RNA polymerases or by a new class of enzymes, the primases (KORNBERG 1980). This mechanism, which can be used in the case of circular DNA molecules or linear DNAs that circularize or form concatemers, cannot be used at the ends of linear DNAs since the RNA primer is removed from the DNA chain, and there is no way of filling the gap resulting at the 5' -ends of the newly synthesized DNA chain. In some cases linear DNA molecules contain a palin dromic nucleotide sequence at the 3' -end that allows the formation of a hairpin structure which provides the needed free 3'-OH group for elongation. This mechanism, first proposed by CAVALIER-SMITH (1974) for eukaryotic DNA repli cation, was shown to take place in several systems (KORNBERG 1980, 1982). Another mechanism to initiate replication consists in the specific nicking of one of the strands of a circular double-stranded DNA, producing a 3'-OH group available for elongation (KORNBERG 1980).

Retroregulation of Bacteriophage ? int Gene Expression.- Tail Length Determination in Double-Stranded DNA Bacteriophages.- Single-Stranded DNA Phage Origins.- Initiation of DNA Replication by Primer Proteins: Bacteriophage ø29 and Its Relatives.

Erscheint lt. Verlag	10.12.2011
Reihe/Serie	Current Topics in Microbiology and Immunology
Zusatzinfo	VII, 90 p.
Verlagsort	Berlin
Sprache	englisch
Maße	170 x 242 mm
Gewicht	195 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Bacteria • Biology • DNA • Elongation • enzymes • Gene • genes • Molecular Biology • Polymer • Protein • proteins • RNA • termination • Virus
ISBN-10	3-642-73117-1 / 3642731171
ISBN-13	978-3-642-73117-4 / 9783642731174
Zustand	Neuware