Human Retroviruses
Humana Press Inc. (Verlag)
978-1-62703-669-6 (ISBN)
Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field.
Virion Attachment & Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand Binding Assay.- CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5α.- The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating.- The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells.- Imaging HIV-1 Nuclear Pre-Integration Complexes.- HIV-1 Reverse Transcription.- RNase H: Specificity, Mechanisms of Action, and Antiviral Target.- HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat.- HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export.- HIV-1 Accessory Proteins: Nef.- HIV-1 Accessory Proteins: VpR.- HIV-1 Accessory Proteins: Vpu and Vif.- SIVSM/HIV-2 Vpx Proteins : Function and Uses in the Infection of Primary Myeloid Cells.- Imaging of HIV Assembly and Release.- HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells.- Determination of HIV-1 Co-Receptor Usage.- The Macrophage and HIV: Basic Concepts and Methodologies.- HIV Infection of Dendritic Cells.- Histocultures (Tissue Explants) in Human Retrovirology.- Single Copy Quantification of HIV-1 in Clinical Samples.- Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells.- HIV-1 Based Lentiviral Vectors.- Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone.- Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques.- Proviral Load Determination of HTLV-1 and HTLV-2 in Patients’ Peripheral Blood Mononuclear Cells by Real-Time PCR.- Quantitative Analysis of HumanT-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real Time RT-PCR (qRT-PCR).
Reihe/Serie | Methods in Molecular Biology ; 1087 |
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Zusatzinfo | 38 Illustrations, color; 24 Illustrations, black and white; XVIII, 345 p. 62 illus., 38 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin |
Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin | |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-62703-669-5 / 1627036695 |
ISBN-13 | 978-1-62703-669-6 / 9781627036696 |
Zustand | Neuware |
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