Current Laboratory Methods in Neuroscience Research

Huangui Xiong, Howard E. Gendelman (Herausgeber)

Buch | Hardcover

585 Seiten

2013
Springer-Verlag New York Inc.
978-1-4614-8793-7 (ISBN)

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Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a “blueprint” for bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.

Section 1: Neurohistological techniques.- 1. Brain tissue preparation, sectioning and staining.- 2. Brain stereotactic injections.- 3. Tract tracing at light and electron microscopy.- 4. Stereological analysis of neurological tissues.- Section 2: In vitro preparations.- 5. Preparation and use of rodent hippocampal brain slices.- 6. Single-Cell Neuronal Dissociation For Electrophysiological Studies.- 7. Isolation and Culture of Human Neurons, Microglia and Astrocytes.- 8. Isolation and Culture of Neural Stem/progenitor Cells.- 9. Isolation of mitochondria from brain tissue and cells.- 10. Isolation of synaptosomes from archived brain tissues.- 11. Xenopus oocytes isolation and microinjection.- Section 3: Leukocyte isolation and application in neuroscience.- 12. Centrifugal elutriation for studies of neuroimmunity.- 13. Flow cytometry in neuroscience research.- Section 4: Standard Laboratory Nucleic Acid and Protein Detections.- 14. Western Blotting Technique in Biomedical Research.- 15. Polymerase chain reaction (PCR) and Real-time RT PCR.- Section 5: Nanomedicine.- 16. Nanoformulations.- 17. Neuronanomedicine.- Section 6: Bioimaging.- 18. Confocal imaging of nerve cells.- 19. Magnetic resonance imaging (MRI).- 20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging.- 21. Non-Invasive Neurophysiological Imaging with Magnetoencephalography.- 22. Multiphoton Brain Imaging.- Section 7: Neuroelectrophysiology.- 23. Techniques for Extracellular Recordings.- 24. “Blind” patch-clamp recordings from rodent hippocampal slices.- 25. Whole-cell patch clamp recordings.- Section 8: Immunohistochemistry and autoradiography.- 26. Analysis of Receptor Binding And Quantitative Autoradiography.- 27. Techniques in Immunohistochemistry and immunocytochemistry.- Section 9: Analysis of Gene Expression.- 28. Protein identification by mass spectrometry: Proteomics.- 29. DNA Microarrays as a Tool for Neurosciences Research.- 30. Metabolomics .- 31.Bioinformatic Methods and Resources for Neuroscience Research.- Section 10: Animal Models: Behavior and Pathology.- 32. Motor function in rodent models of neurodegenerative disorders.- 33. Humanized mice.- 34. Animal models for PD and ALS.- 35. Animal models for Alzheimer’s Disease.- 36. Animal Models: Behavior and Pathology.

Erscheint lt. Verlag	5.11.2013
Reihe/Serie	Springer Protocols Handbooks
Zusatzinfo	189 Illustrations, black and white; XIII, 585 p. 189 illus.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Neurologie
	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	animal behavior • Autoradiography • Bioimaging • immunohistochemistry • In vitro preparations • Leukocyte isolation • Nano-drug delivery • Neuroelectrophysiology • Neurohistological techniques
ISBN-10	1-4614-8793-5 / 1461487935
ISBN-13	978-1-4614-8793-7 / 9781461487937
Zustand	Neuware