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Pharmacogenomics

Methods and Protocols

Federico Innocenti, Ron H.N. van Schaik (Herausgeber)

Buch | Hardcover

359 Seiten

2013 | 2nd ed. 2013
Humana Press Inc. (Verlag)
978-1-62703-434-0 (ISBN)

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Understanding an individual's genetic makeup is the key to creating personalized drugs with greater efficacy and safety, and pharmacogenomics aims to study the complex genetic basis of inter-patient variability in response to drug therapy. Based upon the success of its first edition, the second edition of Pharmacogenomics: Methods And Protocols aims to continue providing readers with high-quality content on the most innovative and commonly adopted technologies in the field of pharmacogenomics as presented by experts in the field. Broken into several sections, this detailed volume examines techniques for interrogating variation in human genes and genomes, functional assessment of genetic variation, both in vitro and in vivo, as well as tools for translation and implementation of pharmacogenetic markers. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thoroughly updated, Pharmacogenomics: Methods And Protocols, Second Edition serves as an essential reference and an invaluable source on the latest information in this field.

Pharmacogenomics: Historical Perspective and Current Status.- Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping.- Clinical SNP Detection by the SmartAmp Method.- MALDI-TOF Mass Spectrometry.- TaqMan® Drug Metabolism Genotyping Assays for the Detection of Human Polymorphisms Involved in Drug Metabolism.- Pyrosequencing of Clinically Relevant Polymorphisms.- Pharmacogenetics Using Luminex® xMAP® Technology: Method for Developing a Custom Multiplex Single Nucleotide Polymorphism Mutation Assay.- Use of Linkage Analysis, Genome-Wide Association Studies, and Next-Generation Sequencing in the Identification of Disease-Causing Mutations.- The GoldenGate Genotyping Assay: Custom Design, Processing, and Data Analysis.- Genome-Wide Gene Expression Profiling, Genotyping, and Copy Number Analyses of Acute Myeloid Leukemia Using Affymetrix GeneChips.- Epigenetic Techniques in Pharmacogenetics.- Plasmid Derived External Quality Controls for Genetic Testing.- Allelic Imbalance Assays to Quantify Allele-Specific Gene Expression and Transcription Factor Binding.- SCAN: A Systems Biology Approach to Pharmacogenomic Discovery.- Methods to Examine the Impact of Nonsynonymous SNPs on Protein Degradation and Function of Human ABC Transporter.- In Vitro Identification of Cytochrome P450 Enzymes Responsible for Drug Metabolism.- In Vitro and In Vivo Mouse Models for Pharmacogenetic Studies.- The Hydrodynamic Tail Vein Assay as a Tool for the Study of Liver Promoters and Enhancers.- A Guide to the Current Web-Based Resources in Pharmacogenomics.- PharmGKB: The Pharmacogenomics Knowledge Base.- Genetic Databases in Pharmacogenomics: The Frequency ofInherited Disorders Database (FINDbase).- Development of Predictive Models for Estimating Warfarin Maintenance Dose Based on Genetic and Clinical Factors.- Evidence Based Drug Dosing and Pharmacotherapeutic Recommendations Per Genotype.

Reihe/Serie	Methods in Molecular Biology ; 1015
Zusatzinfo	33 Illustrations, color; 23 Illustrations, black and white; XIII, 359 p. 56 illus., 33 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie
	Medizin / Pharmazie ► Pharmazie
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	drug therapy • Individual genetic makeup • Personalized medicine • Pharmaceutical design • Pharmacogenomic technologies
ISBN-10	1-62703-434-X / 162703434X
ISBN-13	978-1-62703-434-0 / 9781627034340
Zustand	Neuware