The Eukaryotic Ribosome

H. Bielka (Herausgeber)

Buch | Softcover

338 Seiten

2011 | 1. Softcover reprint of the original 1st ed. 1982
Springer Berlin (Verlag)
978-3-642-68274-2 (ISBN)

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Contents References 11 II. A short historical survey 13 References 16 III. Ribosomes within the cell 20 1. Proportion of ribosomes in tissues 21 2. Free and membrane-bound ribo- mes 22 3. Ribosomes in nucleoli 26 4. Structure of polysomes in the cell 26 5. Ribosomal crystals 33 6. References 33 IV. Some general properties of ribosomes 41 1. Physical characteristics 41 2. Chemical characteristics 43 3. References 44 V. Morphology of ribosomes and poly somes 47 1. Electron microscopy 47 a) The small ribosomal subunit 48 b) The large ribosomal subunit 48 c) The monomeric ribosome 52 d) Three-dimensional models 54 e) Polysomes 55 2. Small-angle X-ray scattering 55 a) The monomeric ribosome 56 b) Polysomes 57 3. References 57 VI. Chemical components 61 1. Ribosomal proteins 61 a) Electrophoretic separation and num ber 61 b) Preparation of single ribosomal pro teins 67 c) Molecular weights 68 d) Amino acid composition and amino acid sequences 71 e) Stoichiometry 71 f) Posttranslational modifications 72 g) Comparison of ribosomal proteins of different tissues 73 h) Species specificities and evolution 74 i) Ribosomal proteins of mitochondria and chloroplasts 75 k) Ribosomal mutants with altered pro teins 75 5 3. Formation of preribosomal 2. Phosphorylation of ribosomal prote particles 139 ins 76 4. References 141 a) In vitro phosphorylation and de phosphorylation 76 VIII. Dissociation - reassociation processes b) In vivo phosphorylation 77 of ribosomal particles 151 c) Phosphorylation of ribosomal pro tein S 6 78 1.

I. Introduction.- References.- A short historical survey.- References.- III. Ribosomes within the cell.- 1. Proportion of ribosomes in tissues.- 2. Free and membrane-bound ribosomes.- 3. Ribosomes in nucleoli.- 4. Structure of polysomes in the cell.- 5. Ribosomal crystals.- 6. References.- IV. Some general properties of ribosomes.- 1. Physical characteristics.- 2. Chemical characteristics.- 3. References.- V. Morphology of ribosomes and polysomes.- 1. Electron microscopy.- 2. Small-angle X-ray scattering.- 3. References.- VI. Chemical components.- 1. Ribosomal proteins.- 2. Phosphorylation of ribosomal proteins.- 3. Immunochemical properties of ribosomal particles and proteins.- 4. Ribosomal ribonucleic acids.- 5. Proteolytic and nucleolytic activities of ribosomal particles.- 6. Other components.- 7. References.- VII. Biosynthesis of ribosomal components and biogenesis of ribosomal particles.- 1. Ribosomal RNA.- 2. Ribosomal proteins.- 3. Formation of preribosomal particles.- 4. References.- VIII. Dissociation-reassociation processes of ribosomal particles.- 1. Dissociation by withdrawal of magnesium.- 2. Other agents for dissociating eukaryotic ribosomes.- 3. Preparation of ribosomal subunits re-associable to active ribosomes.- 4. Reassociation of ribosomal subunits.- 5. Native ribosomal subunits.- 6. References.- IX. Interaction, topography and function of ribosomal components.- 1. Interactions of proteins and RNA.- 2. Topography of proteins and RNA within the ribosome.- 3. Functions of ribosomal proteins.- 4. Functions of ribosomal ribonucleic acids.- 5. References.- X. Interactions of ribosomes with membranes.- 1. Activity of free and membrane-bound polysomes.- 2. Dynamics of ribosomal subunits between free and membrane-bound polysomes.- 3. Chemical properties of free and mem-brane-bound ribosomes.- 4. Components and mechanisms involved in binding of ribosomes to membranes.- 5. Vectorial discharge of polypeptide chains from membrane-bound polysomes.- 6. References.- XI. Function of eukaryotic ribosomes.- 1. Nonribosomal components of the protein synthesizing system.- 2. The process of eukaryotic protein synthesis.- 3. Protein synthesis on membrane-bound ribosomes.- 4. Some regulatory aspects of protein biosynthesis in eukaryotes.- 5. References.- XII. Aphoristic synopsis and outlook.- XIII. Notes added in proof.- XIV. Subject index.- XV. Reference index.

Erscheint lt. Verlag	23.11.2011
Co-Autor	H. Bielka, U. A. Bommer, H. Welfle, F. Noll, P. Westermann
Zusatzinfo	338 p.
Verlagsort	Berlin
Sprache	englisch
Maße	170 x 244 mm
Gewicht	587 g
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Ribosom
ISBN-10	3-642-68274-X / 364268274X
ISBN-13	978-3-642-68274-2 / 9783642682742
Zustand	Neuware