In Living Color
Protocols in Flow Cytometry and Cell Sorting
Seiten
2000
|
1., 2000
Springer Berlin (Verlag)
978-3-540-65149-9 (ISBN)
Springer Berlin (Verlag)
978-3-540-65149-9 (ISBN)
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A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer questions of both new and adept users. This book stems from the editors'experiences managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology.
Introduction.- History.- Getting started.- Sample preparation and cell surface staining.- Reporter genes and cell trackers.- Flow cytometry of nucleic acids.- Intracellular physiological and antibody probes.- Electronic cell sorting.- Core facilities.- Unlocking the spectrum of possibilities.- Appendix.
| Reihe/Serie | Springer Lab Manual |
|---|---|
| Sprache | englisch |
| Maße | 155 x 235 mm |
| Gewicht | 1480 g |
| Einbandart | gebunden |
| Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
| Schlagworte | core facility • flow cytometry • fluoresence activated cell sorting • multiparameter immunofluorescence • reporter gene expression • Zellbiologie • Zelle (Biologie) |
| ISBN-10 | 3-540-65149-7 / 3540651497 |
| ISBN-13 | 978-3-540-65149-9 / 9783540651499 |
| Zustand | Neuware |
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