Methods in Muscle Biology (eBook)

Charles P. Emerson Jr., H. Lee Sweeney (Herausgeber)

eBook Download: PDF

1997 | 1. Auflage
493 Seiten
Elsevier Science (Verlag)
978-0-08-085950-7 (ISBN)

Infos

Methods in Muscle Biology is a comprehensive laboratory guide that details the methods used in the study of muscle biology. The techniques included embrace cell, developmental, and molecular biology, as well asphysiology, neurobiology, and medical research.

Inhalt

Front Cover 1
Methods in Cell Biology: Methods in Muscle Biology 4
Copyright Page 5
Contents 6
Contributors 12
Preface 16
Part I: Embryological Analysis of Myogenesis 18
Chapter 1. Avian Somite Transplantation: A Review of Basic Methods 20
I. Introduction 21
II. Materials 22
III. Methods 28
IV. Summary and Concluding Remarks 42
References 43
Chapter 2. Myogenesis in the Mouse Embryo 46
I. Introduction 46
II. Manipulation of Genes in the Mouse 48
III. Analysis of Gene Expression in the Mouse Embryo 55
IV. Experimental Embryology 60
V. Perspectives 65
References 67
Chapter 3. Myogenesis in Xenopus Embryos 70
I. Fate Maps for Muscle 70
II. Embryo Manipulations 72
III. Molecular Markers of Muscle Differentiation 76
IV. Studies of Xenopus Heart Formation 79
References 80
Chapter 4. Zebrafish: Genetic and Embryological Methods in a Transparent Vertebrate Embryo 84
I. Introduction 85
II. Genetics 85
III. Heart Development 87
IV. Skeletal Muscle Development 89
References 96
Part II: Myogenesis in Cell Culture 100
Chapter 5. Skeletal Muscle Cultures 102
I. Introduction 102
II. Muscle Cell Cultures 103
III. Summary and Future Prospects 130
References 131
Chapter 6. Avian Cardiac Progenitors: Methods for Isolation, Culture, and Analysis of Differentiation 134
I. Establishment of Embryonic Axis 135
II. Pregastrulation Location of Cardiac Progenitors 136
III. Postgastrulation Location of Cardiac Progenitors 136
IV. Removal of Embryos from the Egg 137
V. Removal of Cardiac Progenitors 138
VI. Removal of Endoderm 139
VII. Culturing Whole Embryos 141
VIII. Culturing Explants of Cardiogenic Mesoderm 142
IX. Culturing Primary Cardiomyocytes 143
X. Analysis of Differentiation 144
References 148
Chapter 7. Vascular Smooth Muscle Cell Cultures 150
I. Introduction 150
II. Establishment of Cell Cultures 153
III. Characterization of Cultured Vascular Smooth Muscle Cells 158
IV. Specialized Methods for Human Tissues 164
V. Specialized Methods to Maintain the Differentiated State 167
VI. Conclusions and Perspectives 169
References 170
Chapter 8. Skeletal Muscle Satellite Cell Cultures 172
I. Introduction 172
II. Physiological or Developmental Background of Subjects 174
III. Monolayer Mass Cultures 175
IV. Isolated Single Fiber Cultures 187
V. Advantages and Disadvantages of Each Culture System 190
VI. Conclusions 191
References 192
Part III: Viral and Cellular Gene Delivery Systems in Muscle 194
Chapter 9. Retroviral Gene Delivery 196
I. Introduction 196
II. Vector Construction and Virus Propagation 208
III. Avian Model 213
IV. ALV/Receptor Mouse Model 218
V. Summary/Reprise 224
References 226
Chapter 10. The Use of Replication-Defective Retroviruses for Cell Lineage Studies of Myogenic Cells 232
I. Introduction 232
II. Basic Principles 233
III. Practical Procedures 235
IV. Application of Retroviruses for Muscle Lineage Analyses in Chicken Embryos 240
References 242
Chapter 11. Adenoviral Gene Delivery 246
I. Introduction to Research 247
II. Recombinant Adenovirus Design 251
III. Practical Methods 255
IV. Applications of Adenoviral Gene Transfer to Muscle 258
V. Current Problems and Future Prospects 265
VI. Conclusion 273
References 273
Chapter 12. Methods for Myoblast Transplantation 278
I. Introduction 278
II. Preparation of Cells 279
III. Labeling of Myoblasts in Vitro by Retroviral Mediated Gene Transfer 281
IV. Transplantation Techniques 293
V. Evaluation of Transplant 295
VI. Concluding Remarks 298
References 298
Part IV: Molecular Analysis of Muscle Structure and Function 300
Chapter 13. Structure–Function Analysis of Cytoskeletal/Contractile Proteins in Avian Myotubes 302
I. Introduction 302
II. Muscle Cultures 303
III. Other Considerations 306
References 308
Chapter 14. Functional and Structural Approaches to the Study of Excitation–Contraction Coupling 310
I. Functional and Structural Approaches to the Identification of e–c Coupling Components 311
II. The Dysgenic Muscle Model 323
III. Conclusions 331
References 332
Chapter 15. Adenovirus-Mediated Myofilament Gene Transfer into Adult Cardiac Myocytes 334
I. Introduction 334
II. Isolation of Adult Cardiac Myocytes and Adenovirus-Mediated Gene Transfer 336
III. Production of Recombinant Adenovirus Vectors 340
IV. Analysis of Myofilament Protein Expression and Incorporation in Adenovirus–Infected Ventricular Myocytes 344
V. Summary and Future Directions 347
References 348
Chapter 16. In Vivo Approaches to Neuromuscular Structure and Function 350
1. Introduction 350
II. In Vivo Analysis of Skeletal Muscle and Neuromuscular Innervation 351
III. Manipulation of Motor Neurons, Muscle Fibers, and Synapses in Viuo 364
IV. In Vivo Observations and Manipulations of Muscle Fibers and Neuromuscular Innervation: Practical Considerations 365
V. In Vivo Approaches: New Insights into Neuromuscular Junction Plasticity 366
VI. Summary 373
References 373
Chapter 17. Molecular Diversity of Myofibrillar Proteins: Isoforms Analysis at the Protein and mRNA Level 376
I. Myofibrillar Protein Isoforms in Muscle Tissues 376
II. Multiple Approaches to Isoform Analysis 377
III. Electrophoretic Techniques and Immunoblotting 383
IV. Immunohistochemistry and in Situ Hybridization 386
V. Other Techniques for mRNA Analysis 388
VI. Isoform Analysis and Physiological Studies in Single Muscle Fibers 391
VII. Summary and Perspectives 392
References 394
Part V: Molecular Genetic Analysis of Muscle Gene Regulation 398
Chapter 18. Transgenic Mice: Production and Analysis of Expression 400
I. Introduction 401
II. Productipn of Stable Transgenic Mice 402
III. Production of Transient Transgenics 410
IV. Analysis of Expression at the RNA Level 411
V. Analysis of Expression at the Protein Level 424
VI. Future Directions 427
References 428
Chapter 19. DNA Transfection of Cultured Muscle Cells 432
I. Introduction 432
II. Transient Transfections 433
III. Stable Transfections 439
IV. Identification of cis-Acting Control Regions with Reporter Assays 442
V. Summary and Future Prospects 448
References 449
Chapter 20. DNA- and Adenovirus-Mediated Gene Transfer into Cardiac Muscle 450
I. Introduction 450
II. Methods for Cardiac Injection 453
III. Homogenization of Tissue and Assays 455
IV. Experimental Design 457
V. Future Directions 460
References 462
Chapter 21. Nuclear DNA-Binding Proteins 466
I. Introduction 467
II. Nuclear Extract Preparation: Overview and Important Parameters 468
III. Nuclear Extract Protocol 469
IV. Electromobility Shift Assays: Overview and Important Parameters 473
V. EMSA Protocol 477
VI. DNase I Protection Assays: Overview and Important Parameters 481
VII. DNase I Protection Assay Protocol 485
VIII. Relevance of the EMSA and DNase I Protection Assay to Muscle Research 493
References 498
Index 500
Volumes in Series 516

Produkt-Details

• Erscheint lt. Verlag: 14.11.1997
• Mitarbeit: Herausgeber (Serie): Paul T. Matsudaira, Leslie Wilson
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• ISBN-10: 0-08-085950-X / 008085950X
• ISBN-13: 978-0-08-085950-7 / 9780080859507