Protein NMR Spectroscopy (eBook)

Principles and Practice

John Cavanagh, Wayne J. Fairbrother, Arthur G. Palmer III, Nicholas J. Skelton (Autoren)

eBook Download: PDF

1995 | 1. Auflage
587 Seiten
Elsevier Science (Verlag)
978-0-08-051529-8 (ISBN)

Infos

Protein NMR Spectroscopy: Principles and Practice combines a comprehensive theoretical treatment of high resolution NMR spectroscopy with an extensive exposition of the experimental techniques applicable to proteins and other biological macromolecules. Beginning with simple theoretical models and experimental techniques, Protein NMR Spectroscopy: Principles and Practice develops the complete repertoire of theoretical principals and experimental practices necessary for understanding and implementing the most sophisticated NMR experiments. Protein NMR Spectroscopy: Principles and Practice is written as a graduate-level textbook and will be of particular interest to biochemists, chemists, biophysicists, and structural biologists who utilize NMR spectroscopy as a research tool or who wish to remain abreast of the latest developments in this increasingly important area.

* Special Features:
* First book to combine detailed NMR theory discussions with experimental applications to biomolecules.
* All the theory required to understand these experiments and others.
* Easy to follow progression from a fundamental level to an advanced level.
* Theory of NMR and practical applications for biomolecular investigations presented.
* Theory applied to very practical situations.
* Comprehensive treatment of different levels of theory from simple ideas to density matrix analysis and operator practices.
* Comprehensive description of multi dimensional NMR experiments as applied to unlabeled, 15N-labeled and doubly (13C/15N) labeled proteins.

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Protein NMR Spectroscopy: Principles and Practice combines a comprehensive theoretical treatment of high resolution NMR spectroscopy with an extensive exposition of the experimental techniques applicable to proteins and other biological macromolecules. Beginning with simple theoretical models and experimental techniques, Protein NMR Spectroscopy: Principles and Practice develops the complete repertoire of theoretical principals and experimental practices necessary for understanding and implementing the most sophisticated NMR experiments. Protein NMR Spectroscopy: Principles and Practice is written as a graduate-level textbook and will be of particular interest to biochemists, chemists, biophysicists, and structural biologists who utilize NMR spectroscopy as a research tool or who wish to remain abreast of the latest developments in this increasingly important area.* Special Features: * First book to combine detailed NMR theory discussions with experimental applications to biomolecules.* All the theory required to understand these experiments and others.* Easy to follow progression from a fundamental level to an advanced level.* Theory of NMR and practical applications for biomolecular investigations presented.* Theory applied to very practical situations.* Comprehensive treatment of different "e;levels"e; of theory from simple ideas to density matrix analysis and operator practices.* Comprehensive description of multi dimensional NMR experiments as applied to unlabeled, 15N-labeled and doubly (13C/15N) labeled proteins.

Inhalt

Front cover 1
Title page 4
Copyright page 5
Preface 6
Preface to the First Edition 8
Acknowledgements 12
Table of Contents 14
1 Classical NMR Spectroscopy 28
1.1 Nuclear Magnetism 29
1.2 The Bloch Equations 34
1.3 The One-Pulse NMR Experiment 43
1.4 Linewidth 45
1.5 Chemical Shift 48
1.6 Scalar Coupling and Limitations of the Bloch Equations 50
References 54
2 Theoretical Description of NMR Spectroscopy 56
2.1 Postulates of Quantum Mechanics 56
2.2 The Density Matrix 64
2.3 Pulses and Rotation Operators 77
2.4 Quantum Mechanical NMR Spectroscopy 81
2.5 Quantum Mechanics of Multispin Systems 85
2.6 Coherence 97
2.7 Product Operator Formalism 104
2.8 Averaging of the Spin Hamiltonians and Residual Interactions 129
References 139
3 Experimental Aspects of NMR Spectroscopy 141
3.1 NMR Instrumentation 141
3.2 Data Acquisition 151
3.3 Data Processing 163
3.4 Pulse Techniques 192
3.5 Spin Decoupling 228
3.6 B0 Field Gradients 244
3.7 Water Suppression Techniques 248
3.8 One-Dimensional 1H NMR Spectroscopy 261
References 294
4 Multidimensional NMR Spectroscopy 298
4.1 Two-Dimensional NMR Spectroscopy 300
4.2 Coherence Transfer and Mixing 307
4.3 Coherence Selection, Phase Cycling, and Field Gradients 319
4.4 Resolution and Sensitivity 353
4.5 Three- and Four-Dimensional NMR Spectroscopy 354
References 358
5 Relaxation and Dynamic Processes 360
5.1 Introduction and Survey of Theoretical Approaches 361
5.2 The Master Equation 378
5.3 Spectral Density Functions 392
5.4 Relaxation Mechanisms 397
5.5 Nuclear Overhauser Effect 415
5.6 Chemical Exchange Effects in NMR Spectroscopy 418
References 429
6 Experimental 1H NMR Methods 432
6.1 Assessment of the 1D 1H Spectrum 433
6.2 COSY-Type Experiments 436
6.3 Multiple-Quantum Filtered COSY 464
6.4 Multiple-Quantum Spectroscopy 490
6.5 TOCSY 513
6.6 Cross-Relaxation NMR Experiments 529
6.7 1H 3D Experiments 552
References 556
7 Heteronuclear NMR Experiments 560
7.1 Heteronuclear Correlation NMR Spectroscopy 562
7.2 Heteronuclear-Edited NMR Spectroscopy 608
7.3 13C-13C Correlations: The HCCH-COSY and HCCH-TOCSY Experiments 628
7.4 3D Triple-Resonance Experiments 640
7.5 Measurement of Scalar Coupling Constants 683
7.6 Measurement of Residual Dipolar Coupling Constants 692
References 700
8 Experimental NMR Relaxation Methods 706
8.1 Pulse Sequences and Experimental Methods 707
8.2 Picosecond-Nanosecond Dynamics 712
8.3 Microsecond-Second Dynamics 729
References 748
9 Larger Proteins and Molecular Interactions 752
9.1 Larger Proteins 752
9.2 Intermolecular Interactions 780
9.3 Methods for Rapid Data Acquisition 796
References 802
10 Sequential Assignment, Structure Determination, and Other Applications 808
10.1 Resonance Assignment Strategies 809
10.2 Three-Dimensional Solution Structures 823
10.3 Conclusion 840
References 841
Table of Symbols 846
List of Figures 852
List of Tables 864
Suggested Reading 866
Biomolecular NMR Spectroscopy 866
NMR Spectroscopy 867
Quantum Mechanics 867
Index 868
Spin-1/2 Product Operator Equations 914
Table of Constants 915

Produkt-Details

• Erscheint lt. Verlag: 28.11.1995
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Chemie ► Analytische Chemie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Elektrodynamik
• Themenwelt: Technik
• ISBN-10: 0-08-051529-0 / 0080515290
• ISBN-13: 978-0-08-051529-8 / 9780080515298