Proteome Research (eBook)

Concepts, Technology and Application

M.R. Wilkins, R.D. Appel, K.L. Williams, D.F. Hochstrasser (Herausgeber)

eBook Download: PDF

2007 | 2nd ed. 2007
XXII, 239 Seiten
Springer Berlin (Verlag)
978-3-540-72910-5 (ISBN)

Infos

Proteomics is a multifaceted, interdisciplinary field which studies the complexity and dynamics of proteins in biological systems. It combines powerful separation and analytical technology with advanced informatics to understand the function of proteins in the cell and in the body. This book provides a clear conceptual description of each facet of proteomics, describes recent advances in technology and thinking in each area, and provides details of how these have been applied to a variety of biological problems. It is written by expert practitioners in the field, from industry, research institutions, and the clinic. It provides junior and experienced researchers with an invaluable proteomic reference, and gives fascinating glimpses of the future of this dynamic field.

Inhalt

Preface to Second Edition 6
Foreword to Second Edition 7
Contributors 10
Contents 13
1 Ten Years of the Proteome 19
Abstract 19
1.1 Introduction to the Proteome 19
1.2 Proteomics Is Technology-Driven 21
1.3 What Has Proteomics Delivered? 26
1.4 What Still Eludes Us? 27
1.5 This Book and Some Conclusions 29
References 29
2 Sample Preparation and Prefractionation Techniques for Electrophoresis- Based Proteomics 32
Abstract 32
2.1 Introduction 32
2.2 Conventional Sample Preparation 33
2.3 Artefacts 35
2.4 Multiplexed Approaches to Proteomics 39
2.5 Prefractionation Tools 41
2.6 Other Methods for Prefractionation of Samples 47
2.7 Conclusions 52
References 53
3 Protein Identification in Proteomics 58
Abstract 58
3.1 Introduction 58
3.2 Attributes of Proteins Useful for Their Identification 59
3.3 Protein Identification by Mass Spectrometry 62
3.4 List of Tools and URLs 82
3.5 Concluding Remarks 82
References 83
4 Quantitation in Proteomics 85
Abstract 85
4.1 Introduction 85
4.2 Non-mass-spectrometric Approaches to Quantitation 86
4.3 Relative Quantitation by Mass Spectrometry 90
4.4 Analysis of Known Post-translational Modifications 99
4.5 Conclusions 103
References 104
5 One Gene, Many Proteins 110
Abstract 110
5.1 Introduction 110
5.2 An Overview of Modifications: What Are They and Where Do They Occur? 114
5.3 How Do We Find Post-translational Modifications? 115
5.4 Analysis of Specific Modifications 120
5.5 The Function of Protein Post-translational Modifications: More Than Meets the Eye? 124
5.6 Some Interesting Modification Stories 126
5.7 Future Directions 131
References 131
6 Proteome Imaging 137
Abstract 137
6.1 Introduction 137
6.2 Image Analysis of Two-Dimensional Electrophoresis Gels 138
6.3 Liquid Chromatography–Mass Spectrometry 144
6.4 The Molecular Scanner 148
6.5 Imaging Mass Spectrometry 152
6.6 Conclusion 155
References 156
7 Data Integration in Proteomics 159
Abstract 159
7.1 Introduction 159
7.2 Integration As Gathering and Cross-Linking Information 162
7.3 Integration As Blending of Information 173
7.4 Concluding Remarks 178
References 178
8 Protein–Protein Interactions 183
Abstract 183
8.1 Introduction 183
8.2 Protein–Protein Interactions in Human Diseases: Altered Protein Connectivity Leads to Disorder 184
8.3 Charting Protein–Protein Interactions 186
8.4 Biological and Biomedical Applications 195
8.5 Future Directions 200
References 201
9 Biomedical Applications of Proteomics 207
Abstract 207
9.1 Introduction 207
9.2 The Application of Proteomics to Medicine 208
9.3 Disease Diagnosis from Body Fluids 210
9.4 Vascular Diseases 211
9.5 Neurodegenerative Disorders 216
9.6 Proteomics and Cancer 220
9.7 Toxicopharmacology: the Example of Type 2 Diabetes 225
9.8 Current Limitations and Future Directions of Proteomics for Medicine 229
9.9 Present and Future Directions 231
References 231
10 Proteomics: Where to Next? 236
Abstract 236
10.1 Introduction 236
10.2 The Relevance of -omics to Biology 237
10.3 Technological Developments in Proteomics 238
10.4 The Next Steps for Proteomics: Diagnostics and Drugs 240
10.5 Conclusions 242
References 242
Index 244

Produkt-Details

• Erscheint lt. Verlag: 11.8.2007
• Reihe/Serie: Principles and Practice
• Reihe/Serie: Principles and Practice
• Zusatzinfo: XXII, 239 p.
• Verlagsort: Berlin
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Technik
• Schlagworte: Bioinformatics • bioinformatics protein microanalysis • Bioinformatik • D2 gel electrophoresis • genes • genome project • medical proteomics • protein database • protein post-translational modifications • protein-protein interaction research • proteins • Proteome • Proteome research • Proteomics
• ISBN-10: 3-540-72910-0 / 3540729100
• ISBN-13: 978-3-540-72910-5 / 9783540729105