Coronavirus Replication and Reverse Genetics (eBook)

Luis Enjuanes (Herausgeber)

eBook Download: PDF

2005 | 2005
XI, 257 Seiten
Springer Berlin (Verlag)
978-3-540-26765-2 (ISBN)

Infos

Human coronaviruses caused the SARS epidemic that infected more than 8000 people, killing about ten percent of them in 32 countries. This book provides essential information on these viruses and the development of vaccines to control coronavirus infections.

Inhalt

Preface 5
List of Contents 8
List of Contributors 9
Coronavirus Genome Structure and Replication 10
1 Introduction 11
2 Common Features of Genome Structure Among Coronaviruses 12
3 Cis-Acting RNA Elements in Coronavirus Genome Replication 17
4 Packaging Signals 26
5 Minimum Sequence Requirements for ( Autonomous) Genome Replication 27
6 Importance of Gene Order for Genome Replication 28
7 Future Directions 30
References 31
Coronavirus Transcription: A Perspective 40
1 Introduction 41
2 Discontinuous Transcription by Coronaviruses 44
3 Kinetics of Plus- and Minus-Strand RNA Synthesis and Sensitivity to Translational Inhibition 50
4 The Kinetics of Synthesis of the Subgenomic Minus Strands 51
5 Subgenomic MHV RIs Exist in Infected Cells and Are Transcriptionally Active in mRNA 2–7 Synthesis 52
6 Characterization of Coronavirus Native RI/TIs and Native RF/TFs 53
7 Turnover of MHV Replicative/Transcriptive Intermediates 57
8 A Working Model 59
References 61
The Coronavirus Replicase 66
1 Introduction 67
2 Organization and Expression of the Replicase Gene 68
3 Replicase Polyproteins 70
4 Subcellular Localization of the Coronavirus Replicase 88
5 Concluding Remarks 92
References 92
Viral and Cellular Proteins Involved in Coronavirus Replication 104
1 Introduction 105
2 Viral Proteins in Coronavirus Replication 108
3 Cellular Proteins in Coronavirus Replication 118
4 Perspectives 127
References 128
Coronavirus Reverse Genetics by Targeted RNA Recombination 142
1 Introduction 143
2 Coronavirus RNA Recombination 143
3 Targeted RNA Recombination: Methodology and Technical Issues 146
4 Targeted RNA Recombination: Spectrum of Applications 154
5 Conclusions and Future Prospects 161
References 163
Coronavirus Reverse Genetics and Development of Vectors for Gene Expression 170
1 Introduction 171
2 Pathogenesis Induced by Group 1 Coronaviruses 173
3 Engineering Coronavirus Genome 174
4 Essential Genes Required for TGEV Replication 177
5 Transcription-Regulating Sequences 179
6 Expression Systems Based on Group 1 Coronaviruses 186
7 Coronavirus Vector Cloning Capacity 192
8 Insertion Site, Stability, and Expression Levels 193
9 Molecular Basis of Group 1 Coronavirus Tropism 195
10 Modulation of Coronavirus Vector Virulence 196
11 Biosafety in Coronavirus-Derived Vectors 197
12 Conclusions 198
References 199
Reverse Genetics of Coronaviruses Using Vaccinia Virus Vectors 208
1 Introduction 209
2 The Use of Vaccinia Virus as a Vector for Coronavirus cDNA 211
3 Recombinant Coronaviruses 220
4 Generation of Replicon RNAs 226
5 Development of Coronavirus-Based Multigene Vectors 230
6 Discussion 234
References 235
Development of Mouse Hepatitis Virus and SARS- CoV Infectious cDNA Constructs 238
1 Introduction 239
2 The Coronavirus Genome 239
3 Systematic Approaches to Assembling Coronavirus cDNAs from a Panel of Contiguous Subclones 240
4 Assembling MHV Infectious cDNAs 244
5 SARS-CoV Infectious Clone 254
6 Future Applications 255
References 257
Subject Index 262

Produkt-Details

• Erscheint lt. Verlag: 25.10.2005
• Reihe/Serie: Current Topics in Microbiology and Immunology
• Reihe/Serie: Current Topics in Microbiology and Immunology
• Zusatzinfo: XI, 257 p. 49 illus., 18 illus. in color.
• Verlagsort: Berlin
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Technik
• Schlagworte: Coronavirus Replicase • Diseases • Gene • gene expression • Genetics • genome structure • Hepatitis • Infection • Infections • Mouse Hepatitis Virus • Protein • proteins • RNA • SARS • Transcription-Regulating Sequences • Transmissible Gastroenteritis Coronavirus • Vaccine • Virus
• ISBN-10: 3-540-26765-4 / 3540267654
• ISBN-13: 978-3-540-26765-2 / 9783540267652