Key Determinants of Stress Response in Pseudomonas putida KT2440

Functional genomics of Pseudomonas putida KT2440 and stress sensitive plasposon mutants

Sarah Frank (Autor)

Buch | Softcover

268 Seiten

2015
SÃ¼dwestdeutscher Verlag fÃ¼r Hochschulschriften AG Co. KG
978-3-8381-2523-7 (ISBN)

Titel nicht im Sortiment

Artikel merken

Pseudomonas putida KT2440 is a biosafety strain, which has retained its capability to survive and function in the environment. Its broad metabolic capability to degrade a broad spectrum of aromatic compounds and still grow at temperatures as low as 5°C makes it a good candidate as a model organism to study genome wide responses to changing growth conditions which mimic environmental changes in natural habitats. In this study a functional genomics approach was used to examine the stress response of P. putida KT2440 wild type and five cold sensitive Tn5 mutants (with plasposons inserted in cbrA, cbrB, pcnB, vacB and bipA) to cold shock by decreasing the temperature from 30°C to 10°C. For this, transcriptome data derived from three different transcriptome platforms (Illumina cDNA sequencing, Affymetrix microarrays and Progenika microarrays), as well as metabolome and proteome data were combined. The wild type and a pcaI::Tn5 mutant were furthermore exposed to high concentrations of sodium benzoate (45 mM).

Frank, Sarahgraduated as Dipl.-Ing. in Bioprocessing in Flensburg followed by the degree M.Sc. in Biotechnology in Newcastle upon Tyne, UK. Thereafter she moved to Hannover to work on her PhD, which she successfully completed in 2010. Since then she still works in the field of Pseudomonas research at the Hannover Medical School.

Erscheint lt. Verlag	1.7.2015
Verlagsort	Saarbrucken
Sprache	englisch
Maße	150 x 220 mm
Gewicht	415 g
Themenwelt	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	cDNA sequencing • cold stress • Pseudomonas putida • Transcriptomics
ISBN-10	3-8381-2523-1 / 3838125231
ISBN-13	978-3-8381-2523-7 / 9783838125237
Zustand	Neuware