Permeability Barrier
Humana Press Inc. (Verlag)
978-1-61779-190-1 (ISBN)
The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body. In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute techniques to study this complex system in its many forms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Permeability Barrier: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.
Computational Modeling of the Skin Barrier.- In Vitro Human Skin Segmentation and Drug Concentration-Skin Depth Profiles.- Transcriptional Regulation of Epidermal Barrier Formation.- Epidermal Permeability Barrier (EPB) Measurement in Mammalian Skin.- Assessment of Permeability Barriers to Macromolecules in the Rodent Endometrium at the Onset of Implantation.- Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests.- Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium.- Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells.- Optimization of the Caco-2 Permeability Assay to Screen Drug Compounds for Intestinal Absorption and Efflux.- Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion.- Monitoring of the Dynamics of Epithelial Dome Formation Using a Novel Culture Chamber for Long-Term Continuous Live-Cell Imaging.- Measuring Permeability in Human Retinal Epithelial Cells (ARPE-19): Implications for the Study of Diabetic Retinopathy.- Analysis of Epithelial Barrier Integrity in Polarized Lung Epithelial Cells.- Permeability of Differentiated Human Urothelium In Vitro.- Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry.- An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics.- Analysis of Endothelial Barrier Function In Vitro.- Role of Endothelial Cell-Cell Junctions in Endothelial Permeability.- In Vitro Analyses of Endothelial Cell Permeability.- Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells.- Role of Caveolin-1 in the Regulation of Pulmonary EndothelialPermeability.- Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monolayers.- Permeability of Endothelial Barrier: Cell Culture and In Vivo Models.- Size-Selective and In Vitro Assessment of Inner Blood Retina Barrier Permeability.- Assessment of Permeability in Barrier Type of Endothelium in Brain Using Tracers: Evans Blue, Sodium Fluorescein, and Horseradish Peroxidase.- In Vitro and In Vivo Methods for Assessing FcRn-Mediated Reverse Transcytosis Across the Blood-Brain Barrier.- Evaluation of VEGF-Induced Vascular Permeability in Mice.- In Vivo Measurement of Glioma-Induced Vascular Permeability.- In Vivo Optical Imaging of Ischemic Blood Brain Barrier Disruption.
| Reihe/Serie | Methods in Molecular Biology ; 763 |
|---|---|
| Zusatzinfo | 22 Illustrations, color; 79 Illustrations, black and white; XV, 450 p. 101 illus., 22 illus. in color. With online files/update. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 1-61779-190-3 / 1617791903 |
| ISBN-13 | 978-1-61779-190-1 / 9781617791901 |
| Zustand | Neuware |
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