Medical Mycology Protocols
Humana Press Inc. (Verlag)
978-0-89603-533-1 (ISBN)
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In this text, practitioners describe in full detail numerous methods for working with medically important fungi at the genetic and molecular biology level. The protocols address three main areas of mycological research: the molecular typing and population of fungal pathogens; the molecular aspects and diagnosis of these pathogens; and the cloning and genetic manipulation of pathogenic fungi. The detailed techniques can easily be modified for use with many types of medically important fungi.
Part 1. Opportunistic Fungal Pathogens-An Overview. The Clinical Perspective. The Potential of Molecular Biology. Part 2. General Techniques. Maintenance of Stock Cultures and Species Identification. Culture of Fungal Pathogens. Methods for Fungal DNA and RNA Isolation. Part 3. Molecular Typing and Population Analysis. Generation of Species Specific DNA Probes and Their Applications. DNA Fingerprinting With Minisattelite Probes. Karyotype Analysis of Fungal Populations. RAPD Analysis of Fungal Populations. tRNA Analysis of Fungal Populations. RFLP-Pulsed Field Gel Electrophoretic Analysis of Fungal Populations. PCR Based DNA Fingerprinting (AFLP) for the Analysis of Fungal Populations. Generation of Population Genetic Markers by SWAPP. Molecular Analysis of Nosocomial Infection. Analysis of Fungal Population Structure: A Mathematical Approach. Sequencing of Fungal 16S rRNA Genes. Generation and Analysis of Molecular Phylogenies. Part 4. Molecular Diagnosis of Fungal Infections. Extraction of Fungal DNA From Clinical Samples. PCR for Diagnosis of Fungal Detection. In situ PCR and Hybridization : Investigation of Archival Tissue. ABC Transporters and Antifungal Drug Resistance. Part 5. Cloning and Genetic Manipulation of Fungal DNA and RNA. Currently Available Vectors for Pathogenic Fungi. The pAN Series and Other Positive Selection Vectors. Cloning and Applications of Fungal URA5 Genes. Isolation of ARS Sequences and Plasmid Development. Cloning Genes from Pathogenic Fungi by Complementation. Cloning Genes from Pathogenic Fungi by Reverse Genetics. Cloning of Fungal Chitin Synthases. Cloning of EF-3 Genes by Heterologous Screening. Isolation of Developmental Stage Specific Gene. Differential Display and Subtractive Hybridization. Development of Reporter Systems in Pathogenic Fungi. Mutagenesis of Pathogenic Fungi. Fungal Transposons. Restriction Enzyme Mediated Integration of Pathogenic Fungi. Transformation of Fungi With Exogenous DNA. Gene Disruption in Aspergillus Fumigatus. Gene Disruption in Cryptococcus Neoformans. Gene Disruption in Candida Albicans. Index.
| Reihe/Serie | Methods in Molecular Biology |
|---|---|
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
| Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| ISBN-10 | 0-89603-533-6 / 0896035336 |
| ISBN-13 | 978-0-89603-533-1 / 9780896035331 |
| Zustand | Neuware |
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