Functional Genomics

Michael J. Brownstein, Arkady Khodursky (Herausgeber)

Buch | Softcover

260 Seiten

2010 | Softcover reprint of hardcover 1st ed. 2003
Humana Press Inc. (Verlag)
978-1-61737-474-6 (ISBN)

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This collection of robust, readily reproducible methods for microarray-based studies includes expert guidance in the optimal data analysis and informatics. On the methods side are proven techniques for monitoring subcellular RNA localization en masse, for mapping chromosomes at the resolution of a single gene, and for surveying the steady-state genome-wide distribution of DNA binding proteins in vivo. For those workers dealing with massive data sets, the book discusses the methodological aspects of data analysis and informatics in the design of microarray experiments, the choice of test statistic, and the assessment of observational significance, data reduction, and clustering.

Fabrication of cDNA Microarrays.- Nylon cDNA Expression Arrays.- Plastic Microarrays.- Preparing Fluorescent Probes for Microarray Studies.- Escherichia coli Spotted Double-Strand DNA Microarrays.- Isolation of Polysomal RNA for Microarray Analysis.- Parallel Analysis of Gene Copy Number and Expression Using cDNA Microarrays.- Genome-wide Mapping of Protein-DNA Interactions by Chromatin Immunoprecipitation and DNA Microarray Hybridization.- Statistical Issues in cDNA Microarray Data Analysis.- Experimental Design to Make the Most of Microarray Studies.- Statistical Methods for Identifying Differentially Expressed Genes in DNA Microarrays.- Detecting Stable Clusters Using Principal Component Analysis.- Clustering in Life Sciences.- A Primer on the Visualization of Microarray Data.- Microarray Databases.

"....a good book, and it will be useful to microarray workers in their effots." - Journal of Chemical Neuroanatomy

Erscheint lt. Verlag	9.12.2010
Reihe/Serie	Methods in Molecular Biology ; 224
Zusatzinfo	XII, 260 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-61737-474-1 / 1617374741
ISBN-13	978-1-61737-474-6 / 9781617374746
Zustand	Neuware