Food-Borne Pathogens

Methods and Protocols

Buch | Softcover

268 Seiten

2010 | Softcover reprint of hardcover 1st ed. 2006
Humana Press Inc. (Verlag)
978-1-61737-585-9 (ISBN)

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A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

The Bacteria.- Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources.- Detection and Purification of Bacillus cereus Enterotoxins.- Campylobacter.- Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces.- Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex.- PulseNet’s Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis.- Plesiomonas shigelloides.- Pulsed-Field Gel Electrophoresis As a Molecular Technique in Salmonella Epidemiological Studies.- Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus.- Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples.- HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes.- The Viruses.- Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR.- Detection of Enteroviruses.- Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification.- The Parasites.- Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis.- Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish.- Molecular Identification of Nematode Worms From Seafood (Anisakis spp. and Pseudoterranova spp.) and Meat (Trichinella spp.).- Parallel Studies to the Analysis of Food-Borne Pathogens.- Approaches to Developing Quantitative Risk Assessment Models.- A Review of Surveillance Networks of Food-Borne Diseases.

Erscheint lt. Verlag	19.11.2010
Reihe/Serie	Methods in Biotechnology ; 21
Zusatzinfo	XII, 268 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
ISBN-10	1-61737-585-3 / 1617375853
ISBN-13	978-1-61737-585-9 / 9781617375859
Zustand	Neuware