Embryonic Stem Cell Protocols

Volume II: Differentiation Models

Buch | Softcover

456 Seiten

2010 | Softcover reprint of hardcover 2nd ed. 2006
Humana Press Inc. (Verlag)
978-1-61737-777-8 (ISBN)

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Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

Isolation and Maintenance.- Neural Stem Sphere Method.- Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In Vitro.- Derivation and Characterization of Neural Cells From Embryonic Stem Cells Using Nestin Enhancer.- Optimized Neuronal Differentiation of Murine Embryonic Stem Cells.- Generation of Inner Ear Cell Types From Embryonic Stem Cells.- Derivation of Epidermal Colony-Forming Progenitors From Embryonic Stem Cell Cultures.- Directing Epidermal Fate Selection by a Novel Co-Culture System.- Signaling in Embryonic Stem Cell Differentiation.- In Vitro Generation of T Lymphocytes From Embryonic Stem Cells.- The Role of Hex in Hemangioblast and Hematopoietic Development.- Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells.- Analysis of Embryonic Stem Cell-Derived Osteogenic Cultures.- Generation of Chondrocytes From Embryonic Stem Cells.- Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro.-Generation and Characterization of Cardiomyocytes Under Serum-Free Conditions.- Analysis of Arrhythmic Potential of Embryonic Stem Cell-Derived Cardiomyocytes.- Derivation and Characterization of Alveolar Epithelial Cells From Murine Embryonic Stem Cells In Vitro.- Genetic Manipulation of Embryonic Stem Cells.- Derivation and Characterization of Thyrocyte-Like Cells From Embryonic Stem Cells In Vitro.- Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells.- Formation of Gut-Like Structures In Vitro From Mouse Embryonic Stem Cells.- In Vitro Derivation and Expansion of Endothelial Cells From Embryonic Stem Cells.- Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells.- Integrins and Vascular Development in Differentiated Embryonic Stem Cells In Vitro.- TGF-? Signaling in Embryonic Stem Cell-Derived Endothelial Cells.- Use of Embryonic Stem Cells in Pharmacological and Toxicological Screens.- The Role of the Adapter Protein SHB in Embryonic StemCell Differentiation Into the Pancreatic ?-Cell and Endothelial Lineages.- In Vitro Differentiation of Embryonic Stem Cells Into the Pancreatic Lineage.- Derivation and Characterization of Hepatocytes From Embryonic Stem Cells In Vitro.- Differentiation of Embryonic Stem Cells to Retinal Cells In Vitro.- Epigenetic Analysis of Embryonic Stem Cells.- Derivation and Characterization of Lentoid Bodies and Retinal Pigment Epithelial Cells From Monkey Embryonic Stem Cells In Vitro.- Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-Dimensional Collagen Matrix.

Erscheint lt. Verlag	19.11.2010
Reihe/Serie	Methods in Molecular Biology ; 330
Zusatzinfo	196 Illustrations, black and white; XXII, 456 p. 196 illus.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-61737-777-5 / 1617377775
ISBN-13	978-1-61737-777-8 / 9781617377778
Zustand	Neuware