Gene Delivery to Mammalian Cells

Volume 1: Nonviral Gene Transfer Techniques

Buch | Softcover

301 Seiten

2010 | Softcover reprint of hardcover 1st ed. 2004
Humana Press Inc. (Verlag)
978-1-61737-349-7 (ISBN)

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Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.

Delivery Using Chemical Methods.- Chemical Methods for DNA Delivery.- Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran.- DNA Delivery to Cells in Culture Using Peptides.- DNA Delivery to Cells in Culture Using PNA Clamps.- Dendrimer-Mediated Cell Transfection in Vitro.- DNA Delivery to Cells in Culture Using Cationic Liposomes.- Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium.- Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium.- Delivery of DNA to Tumor Cells Using Cationic Liposomes.- Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes.- Delivery Using Physical Methods.- Gene Delivery Using Physical Methods.- Gene Delivery to Mammalian Cells by Microinjection.- Delivery of DNA to Cells in Culture Using Particle Bombardment.- Delivery of DNA to Skin by Particle Bombardment.- Biolistic Transfection of Cultured Organotypic Brain Slices.- Efficient Electroporation of Mammalian Cells in Culture.- Delivery of DNA to Skin by Electroporation.- In Vivo DNA Electransfer in Skeletal Muscle.- Electrically Mediated Plasmid DNA Delivery to Solid Tumors In Vivo.- Hydrodynamic Delivery of DNA.- Naked DNA Gene Transfer in Mammalian Cells.- Microparticle Delivery of Plasmid DNA to Mammalian Cells.- DNA Delivery to Cells in Culture Using Ultrasound.- DNA Delivery to Cells In Vivo by Ultrasound.

Erscheint lt. Verlag	10.11.2010
Reihe/Serie	Methods in Molecular Biology ; 245
Zusatzinfo	XV, 301 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-61737-349-4 / 1617373494
ISBN-13	978-1-61737-349-7 / 9781617373497
Zustand	Neuware