RT-PCR Protocols
Humana Press Inc. (Verlag)
978-1-60761-628-3 (ISBN)
User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.
The RT-PCR Detective: Hunting Down the Best Method.- Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology.- Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer.- Transcriptome Profiling of Host–Microbe Interactions by Differential Display RT-PCR.- Quantitative RT-PCR Methods for Mature microRNA Expression Analysis.- Detection of Influenza A Virus Neuraminidase and PB2 Gene Segments by One Step Reverse Transcription Polymerase Chain Reaction.- Detection and Identification of CD46 Splicing Isoforms by Nested RT-PCR.- Simultaneous Detection of Bluetongue Virus RNA, Internal Control GAPDH mRNA, and External Control Synthetic RNA by Multiplex Real-Time PCR.- Detection of West Nile Viral RNA from Field-Collected Mosquitoes in Tropical Regions by Conventional and Real-Time RT-PCR.- Detection of Antisense RNA Transcripts by Strand-Specific RT-PCR.- RT-PCR Amplification and Cloning of Large Viral Sequences.- The RT-PCR Mathematician: Assessing Gene and RNA Expression.- One-Step RT-LATE-PCR for mRNA and Viral RNA Detection and Quantification.- Changes in Gene Expression of Caveolin-1 After Inflammatory Pain Using Quantitative Real-Time PCR.- Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction.- The Use of Comparative Quantitative RT-PCR to Investigate the Effect of Cysteine Incubation on GPx1 Expression in Freshly Isolated Cardiomyocytes.- The Renaissance of Competitive PCR as an Accurate Tool for Precise Nucleic Acid Quantification.- The RT-PCR Master Chef: Finding the Right Ingredients.- Skeletal Muscle RNA Extraction in Preparation for RT-PCR.- Reverse Transcription of the Ribonucleic Acid: The First Step in RT-PCR Assay.- Primer Design for RT-PCR.- Hot Start PCR.- Real-time RT-PCR for Automated Detection ofHIV-1 RNA During Blood Donor Screening.
Reihe/Serie | Methods in Molecular Biology ; 630 |
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Zusatzinfo | XI, 341 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Schlagworte | Cloning • Gene sequences • Primer design • Real-time quantitative RT-PCR • Reverse transcription • Reverse-transcription polymerase chain reaction |
ISBN-10 | 1-60761-628-9 / 1607616289 |
ISBN-13 | 978-1-60761-628-3 / 9781607616283 |
Zustand | Neuware |
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