G ProteinsTechniques of Analysis
Crc Press Inc (Verlag)
978-0-8493-3381-1 (ISBN)
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Incorporating a bench-top format, G Proteins: Techniques of Analysis covers essential methods - with a commitment to those techniques of proven and current utility.
G Proteins: Techniques of Analysis includes expression and functional analysis of G proteins; evaluation of covalent modifications and other regulatory phenomena; and, mapping pathways established among receptors, G proteins, and effectors.
Including contributors from key institutions, each author offers clear instructions to establish a synthesized, concise and consistent approach to each chapter, beneficial to students and professionals as well.
David R. Manning, Ph.D. Department of Pharmacology University of Pennsylvania School of Medicine Philadelphia, PA
Preface Expression and Purification of G Protein a Subunits in E. scherichia coli - M. Linder Purification of Recombinant G Protein a and bg Subunits from Sf9 Cells-T. Kozasa Expression and Functional Analysis of G Protein a Subunits in Mammalian Cells...-C. H. Berlot Identification of Regulators and Targets of G Protein a Subunits Using the Yeast Two-Hybrid System-J.L. Glick, T. E. Meigs, and P. J. Casey Functional Analysis of G Protein bg Subunits...-K. Yan, O. Kisselev, A. Pronin and N. Gautam Immunological Techniques of Analysis-C. A. Chen and D. R. Manning GTPase-Activating Proteins (GAPs) for Heterotrimeric G Proteins- J. Wang, Y. Tu, S. Mukhopadhyay, P. Chidiac, G. Biddlecome and E. M. Ross Fatty Acid Acylation of G Protein a Subunits- P.B.Wedegaetner ADP-Ribosylation of G Proteins with Cholera Toxin or Pertussis Toxin- M. J. Woolkalis Analysis of Receptor.G Protein coupling with PTX-Risistant Analogues of G(i in subscript) and G(o in subscript)- A. Mortiz and R. Taussig Microinjection of Antibodies Neutralizing G. Protein Function-V. J. LaMorte, J. R. Feramisco and J. L. Meinkoth Agonist-Promoted (35S)GTPgS-Binding as a Probe of Receptor-G Protein Communication in Reconstituted Sf9 Cells- A.J. Barr and D. R. Manning Methods of Sequestering bg in the Intact Cell- G. J. Della Rocca, R. J. Lefkowitz and W. J. Koch In Vitro and In Vivo Analysis of Heterotrimeric G-Protein Signaling via DNA/RNA Antisense Strategies-H. Wang, X. Liu, Z. Cheng, H. Ling Li and C. C. Malbon
| Erscheint lt. Verlag | 19.5.1999 |
|---|---|
| Reihe/Serie | Methods in Signal Transduction Series |
| Zusatzinfo | 40 Tables, black and white; 37 Halftones, black and white |
| Verlagsort | Bosa Roca |
| Sprache | englisch |
| Maße | 156 x 234 mm |
| Gewicht | 566 g |
| Einbandart | gebunden |
| Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Pharmakologie / Toxikologie |
| Naturwissenschaften ► Biologie ► Biochemie | |
| ISBN-10 | 0-8493-3381-4 / 0849333814 |
| ISBN-13 | 978-0-8493-3381-1 / 9780849333811 |
| Zustand | Neuware |
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