Microarrays in Clinical Diagnostics

Thomas O. Joos, Paolo Fortina (Herausgeber)

Buch | Hardcover

288 Seiten

2005
Humana Press Inc. (Verlag)
978-1-58829-394-7 (ISBN)

Artikel merken

Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Noncontact Laser Microdissection and Pressure Catapulting.- Applications of the Universal DNA Microarray in Molecular Medicine.- Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization.- Genotyping Single-Nucleotide Polymorphisms by Minisequencing Using Tag Arrays.- Single-Nucleotide Polymorphism and Mutation Identification by the Nanogen Microelectronic Chip Technology.- Molecular Diagnostic Testing for Inherited Thrombophilia Using Invader®.- Controlled Agitation During Hybridization.- Rapid Screening for 31 Mutations and Polymorphisms in the Cystic Fibrosis Transmembrane Conductance Regulator Gene by Luminex® xMAP™ Suspension Array.- Protein Microarray-Based Screening of Antibody Specificity.- Multiplexed Protein Analysis Using Antibody Microarrays and Label-Based Detection.- Allergen Microarrays.- Enhanced Protein Profiling Arrays for Quantitative Measurement of Protein Expression in Multiple Samples.- Multiplexed Cytokine Sandwich Immunoassays Clinical Applications.- Validation and Quality Control of Protein Microarray-Based Analytical Methods.- Tissue Microarrays.

Erscheint lt. Verlag	1.6.2005
Reihe/Serie	Methods in Molecular Medicine ; 114
Zusatzinfo	1 Illustrations, color; 95 Illustrations, black and white; 288 p. 96 illus., 1 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 2. Studienabschnitt (Klinik) ► Anamnese / Körperliche Untersuchung
	Studium ► 2. Studienabschnitt (Klinik) ► Pathologie
ISBN-10	1-58829-394-7 / 1588293947
ISBN-13	978-1-58829-394-7 / 9781588293947
Zustand	Neuware