Chemogenomics

Methods and Protocols

Daniel Merk, Apirat Chaikuad (Herausgeber)

Buch | Softcover

234 Seiten

2024 | 2023 ed.
Springer-Verlag New York Inc.
978-1-0716-3399-1 (ISBN)

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This volume presents both theoretical guidance and protocols on chemogenomics including chemogenomics library assembly, compound profiling, and phenotypic assays. The chapters in this book cover topics such as the assembly and use of Kinase Chemogenomics; data mining for chemogenomic compound candidates; protocols for protein family-focused assay systems to profile chemogenomic compounds; functional and target engagement assays in cellular settings for broad characterization; and a discussion on phenotypic assays where chemogenomic sets may be applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Chemogenomics: Methods and Protocols is a valuable resource for all researchers whoare interested in learning more about this diverse and developing field.

An Introduction to Chemogenomics.- Developing a Kinase Chemogenomic Set: Facilitating Investigation into Kinase Biology by Linking Phenotypes to Targets.- Compilation of Custom Compound/Bioactivity Datasets from Public Repositories.- Quality Control of Chemogenomic Library using LC-MS.- Annotation of the Effect of Chemogenomic Compounds on Cell Health using High-Content Microscopy in Live-Cell Mode.- Characterization of Cellular Viability using Label-Free Brightfield Live-Cell Imaging.- Plate-Based Screening for DUB Inhibitors.- NanoBRET(TM) Live Cell Kinase Selectivity Profiling Adapted for High Throughput Screening.- A Fluorescence-Based Reporter Gene Assay to Characterize Nuclear Receptor Modulators.- Measuring Protein-Protein Interactions in Cells using NanoLuciferase Bioluminescence Resonance Energy Transfer (NanoBRET) Assay.- HiBiT Cellular Thermal Shift Assay (HiBiT CETSA).- Detection of Cellular Target Engagement for Small Molecule Modulators of Striatal-Enriched Protein Tyrosine Phosphatase (STEP).- Target Deconvolution by Limited Proteolysis Coupled to Mass Spectrometry.- Global Assessment of Drug Target Engagement and Selectivity of Covalent Cysteine-Reactive Inhibitors using Alkyne-Functionalized Probes.- 3D-Spheroid Invasion Assay for High-Throughput Screening of Small Molecule Libraries.- Live-Cell High-Throughput Screen for Monitoring Autophagy Flux.- Phenotypic Chemical Screening in CD4+ T Cells to Identify Epigenetic Inhibitors.

Erscheinungsdatum	21.08.2024
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	60 Illustrations, color; 2 Illustrations, black and white; XI, 234 p. 62 illus., 60 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	Annotated chemogenomic compounds • Druggable human proteome • Drug targets • novel drugs • Targeted chemical libraries • Target validation
ISBN-10	1-0716-3399-6 / 1071633996
ISBN-13	978-1-0716-3399-1 / 9781071633991
Zustand	Neuware