Epidermal Growth Factor

Methods and Protocols

Tarun B. Patel, Paul J. Bertics (Herausgeber)

Buch | Hardcover

208 Seiten

2005
Humana Press Inc. (Verlag)
978-1-58829-421-0 (ISBN)

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A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

A Historical Perspective of the EGF Receptor and Related Systems.- Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromatography.- Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay.- Analysis of EGF Receptor Interactions With the ? Subunit of the Stimulatory GTP Binding Protein of Adenylyl Cyclase, Gs.- Regulator of Epidermal Growth Factor Signaling.- Dissecting the Epidermal Growth Factor Receptor Signal Transactivation Pathway.- A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptor.- In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases.- Assays to Monitor Degradation of the EGF Receptor.- Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members.- Epithelial Cell Migration in Response to Epidermal Growth Factor.- Motility Signaled From the EGF Receptor and Related Systems.- Methods for Determining the Proliferation of Cells in Response to EGFR Ligands.- Clinical Advancement of EGFR Inhibitors in Cancer Therapy.

Erscheint lt. Verlag	15.12.2005
Reihe/Serie	Methods in Molecular Biology ; 327
Zusatzinfo	1 Illustrations, color; 45 Illustrations, black and white; XII, 208 p. 46 illus., 1 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-58829-421-8 / 1588294218
ISBN-13	978-1-58829-421-0 / 9781588294210
Zustand	Neuware