Measles and Related Morbilliviruses

Methods and Protocols

Dzwokai Z. Ma, Christian K. Pfaller (Herausgeber)

Buch | Hardcover

266 Seiten

2024 | 2024 ed.
Springer-Verlag New York Inc.
978-1-0716-3869-9 (ISBN)

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This volume covers the latest protocols used to study the molecular biology of morbilliviruses, including cell entry, replication, and virus-host interactions. The chapters in this book also cover methods for studying morbillivirus infection through the use of novel tissue culture and in vivo models; analyzing the biological implications of infection by using primary tissues isolated from infected animals; and exploring diagnostic tools to evaluate humoral immunity against human-pathogenic measles virus (MeV). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Measles and Related Morbilliviruses: Methods and Protocols is a valuable resource for researchers studying MeV,and will be useful to researchers studying other members of Paramyxoviridae and Mononegavirales.

A Quantitative Fusion Assay to Study Measles Virus Entry.- A Semi-Quantitative Protein-Fragment Complementation Assay to Study Protein-Protein Interactions of the Polymerase Complex In Cellula.- Design and Execution of In Vitro Polymerase Assays for Measles Virus and Related Mononegaviruses.- Optical Control of Mononegavirus Gene Expression and Replication.- Generation of Defective Interfering Particles of Morbilliviruses Using Reverse Genetics.- Molecular Analysis of Copy Back Defective Interfering RNAs of Morbilliviruses.- Applying Reverse Genetics to Study Measles Virus Interactions with the Host.- Identification of Host Factors of Paramyxoviruses by siRNA Genome-Wide Screens.- Dual RNA-seq Analysis of Patients' Cells and Viral Genome After Measles Infection.- Investigating Liquid-Liquid Phase Separation in Virus-Generated Inclusion Bodies Using Fluorescence Recovery After Photobleaching of Fluorescently Labeled Host Proteins.- Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection.- In Vitro Modeling of Feline Morbillivirus Infections Using Primary Feline Kidney Cells.- Setup of Aerosol Delivery System to Prevent Measles Virus Infection in Nonhuman Primate Model.- Histopathological Analysis of Brains from Dogs Infected with Canine Distemper Virus.- Canine Distemper Virus Pathogenesis in the Ferret Model.- Measles Foci Reduction Neutralization Test (FRNT).- Multiplex Bead Assay for Serological Surveillance of Measles and Rubella.- Measles IgG Avidity Assay.

Erscheinungsdatum	15.05.2024
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	3 Illustrations, black and white; XIV, 266 p. 3 illus.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	antivirals • Negative Strand RNA Viruses • Paramyxoviridae family • Reverse Genetics • Vaccine development • viral life cycle
ISBN-10	1-0716-3869-6 / 1071638696
ISBN-13	978-1-0716-3869-9 / 9781071638699
Zustand	Neuware