Microfluidics Diagnostics

Methods and Protocols

Valérie Taly, Stéphanie Descroix, Karla Perez-Toralla (Herausgeber)

Buch | Hardcover

255 Seiten

2024 | 2024 ed.
Springer-Verlag New York Inc.
978-1-0716-3849-1 (ISBN)

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This detailed volume explores a wide range of microfluidic-based approaches that exploit the unique features of microfluidic devices, which hold significant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell-based culture and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microfluidics Diagnostics: Methods and Protocols serves as an ideal guide for researchers investigating how microfluidics can shape the future of diagnosis.

Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and Nucleic Acids.- DNA Sizing with pg/mL Sensitivity for Cell-Free DNA Analysis with the mLAS Technology.- On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samples.- Isolation of Extracellular Vesicles from Cell Culture and Blood through Nano-Targeted DLD Microfluidic Device.- Microchip for immunomagnetic sorting of Circulating Tumor Cells (CTCs).- Enhancing the Efficiency of Conventional Surface Immunoassays within Standard Labware Using Microscale Flows.- Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis.- Microfluidic Cartridge for Bead-Based Affinity Assays.- A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level.- Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets.- A Thermoplastic Microsystem to Perform Antibiotic Susceptibility Testing by Monitoring Oxygen Consumption.- An Immiscible-Phase Filtration Device for Isolation, Amplification, and Detection of Nucleic Acids for Clinical Diagnostics.- Microfluidic 3D Cytotoxic Assay.- Evaluating Nanoparticles Penetration by a New Microfluidic Hydrogel-Based Approach.- Selective Targeting of Tumor Cells in a Microfluidic Tumor Model with Multiple Cell Types.

Erscheinungsdatum	18.05.2024
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	70 Illustrations, color; 4 Illustrations, black and white; XII, 255 p. 74 illus., 70 illus. in color. With online files/update.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Anamnese / Körperliche Untersuchung
	Naturwissenschaften ► Chemie ► Analytische Chemie
	Technik ► Elektrotechnik / Energietechnik
	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	Bacteria and virus analysis • Biomarkers • Diagnosis-based microdevices • Microfluidic Devices • sample preparation
ISBN-10	1-0716-3849-1 / 1071638491
ISBN-13	978-1-0716-3849-1 / 9781071638491
Zustand	Neuware