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Pharmacogenomics

Methods and Protocols

Federico Innocenti (Herausgeber)

Buch | Hardcover

214 Seiten

2005
Humana Press Inc. (Verlag)
978-1-58829-440-1 (ISBN)

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Expert researchers and physician/clinicians describe in detail the newest and most commonly used technologies today in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromarography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions.

History and Overview.- Pharmacogenomics.- Functional Analysis of Gene Variation.- Transfection Assays With Allele-Specific Constructs.- Snapshot of the Allele-Specific Variation in Human Gene Expression.- Genome-Wide Analysis of Allele-Specific Gene Expression Using Oligo Microarrays.- HaploChIP.- Genotyping Techniques.- Aspects Influencing Genotyping Method Selection.- Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping.- Pyrosequencing of Clinically Relevant Polymorphisms.- Kinetic Fluorescence-Quenching Detection Assay for Allele Frequency Estimation.- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.- Fluorescence-Based Fragment Size Analysis.- Single-Nucleotide Polymorphism Genotyping in DNA Pools.- TaqMan Genotyping of Insertion/Deletion Polymorphisms.- Management of Pharmacogenomic Information.- PharmGKB.- Systems for the Management of Pharmacogenomic Information.

Erscheint lt. Verlag	15.8.2005
Reihe/Serie	Methods in Molecular Biology ; 311
Zusatzinfo	X, 214 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie
Themenwelt	Medizin / Pharmazie ► Pharmazie ► PTA / PKA
ISBN-10	1-58829-440-4 / 1588294404
ISBN-13	978-1-58829-440-1 / 9781588294401
Zustand	Neuware