RNA Vaccines

Methods and Protocols

Buch | Hardcover

389 Seiten

2024 | 2nd ed. 2024
Springer-Verlag New York Inc.
978-1-0716-3769-2 (ISBN)

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This second edition volume expands on the previous edition with discussions of the latest technology and advancements in the development of RNA vaccines and proposed solutions on how to tackle currently unmet medical needs. The chapters in this book are organized into four parts and cover topics such as replication and non-replicating RNA vectors, formulation and delivery of RNA vaccines, and clinical and nonclinical development of RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, RNA Vaccines: Methods and Protocols, Second Edition is a valuable resource for any researcher interested in learning more about this important and developing field.

Introduction to RNA Vaccines Post COVID-19.- Self-Replicating RNA Derived from the Genomes of Positive Strand RNA Viruses.- Negative Strand RNA Virus Vectored Vaccines.- New Generation Self-Replicating RNA Vaccines Derived from Pestivirus Genome.- Trans-Amplifying RNA Vaccines Against Infectious Diseases: A Comparison with Non-Replicating and Self-Amplifying RNA.- Generation and Characterization of In Vitro Transcribed mRNA.- Production and Evaluation of Nucleoside-Modified mRNA Vaccines for Infectious Diseases.- Sequence-Optimized mRNA Vaccines Against Infectious Disease.- Design and Synthesis of Circular RNA Expression Vectors.- Electroporation of mRNA as a Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins.- A Basic Method for Formulating mRNA-Lipid Nanoparticle Vaccines in the Lab.- Messenger RNA Lipid-Based Nanoparticles: Optimization of Formulations in the Lab.- Plant Expression of Trans-Encapsidated Chimeric Viral Vaccines with Animal RNA Replicons: An Update.- Producing Plasmid DNA Template for Clinical Grade RNA Vaccine Manufacture.- Regulatory Considerations for Producing mRNA Vaccines for Clinical Trials.- Quality by Digital Design for Developing Platform RNA Vaccine Manufacturing Processes.- Nonclinical Safety Testing of RNA Vaccines.

Erscheinungsdatum	31.05.2024
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	49 Illustrations, color; 8 Illustrations, black and white; XII, 389 p. 57 illus., 49 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	Adjuvant activity • clinical development • Preclinical development • RNA vectors • Vaccine Delivery
ISBN-10	1-0716-3769-X / 107163769X
ISBN-13	978-1-0716-3769-2 / 9781071637692
Zustand	Neuware