Platelets and Megakaryocytes

Functional Assays

Jonathan M. Gibbins, Martyn P. Mahaut-Smith (Autoren)

Jonathan M. Gibbins, Martyn P. Mahaut-Smith (Herausgeber)

Software / Digital Media

450 Seiten

2004
Humana Press Inc. (Hersteller)
978-1-59259-782-6 (ISBN)

Infos

Platelets are crucial for hemostasis, yet excessive platelet activation is a major cause of morbidity. Understanding megakaryocyte biology is crucial for the development of platelet gene targeting and these two volumes bring together those techniques for the study of both the platelet and the megakaryocyte. Volume 1 covers functional assays including aggregometry, flow cytometry, as well as approaches used to study megakaryocyte function. Volume 2 includes signaling techniques, postgenomic methods and a number of key perspectives chapters that review our current understanding of platelet and megakaryocyte biology in addition to new developments in experimental strategies making these essential books for all researchers in this field.

Inhalt

Part I. Platelet Functional Assays; 1 Effects of Anticoagulants Used During Blood Collection on Human Platelet Function Jane May and Stanley Heptinstall; 2 Preparation of Washed Platelet Suspensions from Human and Rodent Blood Jean-Pierre Cazenave, Philippe Ohlmann, Dominique Cassel, Anita Eckley, Beatrice Hechler, and Christian Gachet; 3 Platelet Counting Paul Harrison, Carol Briggs, and Samuel J. Machin; 4 Electron Microscopy Methods for Studying Platelet Structure and Function James G. White; 5 Platelet Aggregation in Whole Blood: Impedance and Particle Counting Methods Gavin E. Jarvis; 6 Platelet Aggregation: Turbidimetric Measurements Gavin E. Jarvis; 7 Secretion From Dense Granules. Luminescence Method for Adenine Nucleotides M. Fred Heath; 8 Platelet Dense Granule Secretion: the [3H]-5-HT Secretion Assay David Crosby and Alastair W. Poole; 9 Analysis of Releasable Nucleotides of Platelets Joachim Jankowski, Lars Henning, and Hartmut Schluter; 10 Studies of Secretion Using Permeabilized Platelets Tara W. Rutledge and Sidney W. Whiteheart; 11 Measurement of Platelet Arachidonic Acid Metabolism Richard W. Farndale, Philip G. Hargreaves, Joanna L. Dietrich, and Rosemary J. Keogh; 12 Measurement of Platelet Procoagulant Response Johan W. M. Heemskerk, Paul Comfurius, Marion A. H. Feijge, and Edouard M. Bevers; 13 Platelet Adhesion Assays Performed Under Static Conditions Joanne M. Stevens; 14 Platelet Adhesion Assays Under Flow Using Matrix Protein-Coupled Adhesion Columns Renata Polanowska-Grabowska and Adrian R. L. Gear; 15 Techniques to Examine Platelet Adhesive Interactions Under Flow Suhasini Kulkarni, Warwick S. Nesbitt, Sacha M. Dopheide, Sascha C. Hughan, Ian S. Harper, and Shaun P. Jackson; 16 In vivo Models of Platelet Function and Thrombosis: Study of Real-Time Thrombus Formation Shahrokh Falati, Peter L. Gross, Glenn Merrill-Skoloff, Derek Sim, Robert Flaumenhaft, Alessandro Celi, Barbara C. Furie, and Bruce Furie; 17 Adhesion Between Platelets and Leukocytes or Endothelial Cells Gerard B. Nash; 18 In vitro Measurement of High Shear Platelet Adhesion and Aggregation by the PFA-100R Paul Harrison; 19 Flow Cytometric Analysis of Platelet Membrane Glycoprotein Expression and Platelet Activation Alison H. Goodall and Jackie Appleby; 20 Flow Cytometric Analysis of Mouse Platelet Function Bernhard Nieswandt, Valerie Schulte, and Wolfgang Bergmeier; 21 Platelet Microparticles Shosaku Nomura and Shirou Fukuhara; Part II. Megakaryocyte Functional Assays; 22 Isolation of Primary Megakaryocytes and Studies of Proplatelet Formation Robert M. Leven; 23 Isolation and Culture of Megakaryocyte Precursors Najet Debili, Fawzia Louache, and William Vainchenker; 24 Assays of Megakaryocyte Development: Surface Antigen Expression, Ploidy, and Size Anthony Mathur, Ying Hong, Guosu Wang, and Jorge D. Erusalimsky; 25 Assays of Megakaryocyte Development: Cytoplasm, Storage Granules, and Demarcation Membranes Arnaud Drouin, Gulie Alimardani, and Elisabeth Cramer; 26 Thrombopoietin Bioassay Warren S. Alexander and Craig Hyland; 27 Culture of Megakaryocytic Cell Lines: Uses and Limitations Norio Komatsu

Produkt-Details

• Reihe/Serie: Methods in Molecular Biology ; 272
• Verlagsort: Totowa, NJ
• Sprache: englisch
• Themenwelt: Medizinische Fachgebiete ► Innere Medizin ► Hämatologie
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• ISBN-10: 1-59259-782-3 / 1592597823
• ISBN-13: 978-1-59259-782-6 / 9781592597826
• Zustand: Neuware