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Human Retrovirus Protocols

Virology and Molecular Biology

Tuofu Zhu (Herausgeber)

Buch | Hardcover

494 Seiten

2005
Humana Press Inc. (Verlag)
978-1-58829-495-1 (ISBN)

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A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.

Methods for Virus Isolation and Detection.- Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals.- Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals.- Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes.- Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived Macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid.- Isolation and HIV-1 Infection of Primary Human Microglia From Fetal and Adult Tissue.- Isolation of Human Immunodeficiency Virus Type 1 From Semen and Vaginal Fluids.- Isolation and Quantification of HIV From Lymph Nodes.- Isolation of Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes.- Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells.- Isolation of Foamy Viruses From Peripheral Blood Lymphocytes.- Alu-LTR Real-Time Nested PCR Assay for Quantifying Integrated HIV-1 DNA.- Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR.- Detection of HIV-1 Provirus and RNA by In Situ Amplification.- Detection of HTLV-1 Gene on Cytologic Smear Slides.- Detection of HIV-2 by PCR.- Quantitation of HIV-1 Viral RNA in Blood Plasma and Genital Secretions.- Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR.- Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR.- A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay.- A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity.- Detection of Antiviral Antibodies Using Enzyme-Linked Immunosorbent Assay.- Plaque-Reduction Assays for Human and Simian Immunodeficiency Virus Neutralization.- Detection of Drug-Resistant HIV-1 Strains.- Molecular Biology Methods.- Determination of Cell Tropism of HIV-1.- Determination of Co-Receptor Usage of HIV-1.- Quantitative Evaluation of HIV and SIV Co-Receptor Use With GHOST(3) Cell Assay.- Phenotypic Characterization of Blood Monocytes From HIV-Infected Individuals.- Methods to Determine HIV-1 Ex Vivo Fitness.- A Yeast Recombination-Based Cloning System to Produce Chimeric HIV-1 Viruses and Express HIV-1 Genes.- PCR Amplification, Cloning, and Construction of HIV-1 Infectious Molecular Clones From Virtually Full-Length HIV-1 Genomes.- Amplification and Cloning of Near Full-Length HIV-2 Genomes.- Growth and Manipulation of a Human T-Cell Leukemia Virus Type 2 Full-Length Molecular Clone.- Construction and Analysis of Genomic, Full-Length Infectious Foamy Virus DNA Clones.- Molecular Characterization of Proteolytic Processing of the Gag Proteins of Human Spumaretrovirus.- Assessing the Relative Efficacy of Antiretroviral Activity of Different Drugs on Macrophages.- Gene Expression Profiling of HIV-1 Infection Using cDNA Microarrays.- Determination of DC-SIGN and DC-SIGNR Repeat Region Variations.

Erscheint lt. Verlag	17.5.2005
Reihe/Serie	Methods in Molecular Biology ; 304
Zusatzinfo	XIV, 494 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
ISBN-10	1-58829-495-1 / 1588294951
ISBN-13	978-1-58829-495-1 / 9781588294951
Zustand	Neuware