Hypoxia

Methods and Protocols

Buch | Hardcover

266 Seiten

2024
Springer-Verlag New York Inc.
978-1-0716-3632-9 (ISBN)

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This volume explores the latest ways to detect hypoxia in the context of cancer, including techniques to study gene expression changes, protein responses, and cellular adaptations to low-oxygen conditions. This book also covers new protocols to characterize hypoxia in tumors with high spatial resolution and novel therapeutic approaches that consider the complex microenvironments of solid tumors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Hypoxia: Methods and Protocols is a valuable tool to help cancer researchers and other scientists detect hypoxia in cancer and other disease pathologies.

Preface…
Table of Contents…
Contributing Authors…

Part I Fluorescent Reporter Methods for Hypoxia Detection

1.     Genetically Encoded Reporters to Monitor Hypoxia
Nadine Bauer and Friedemann Kiefer

2.     Detection of Hypoxia in 2D and 3D Cell Culture Systems using Genetically Encoded Fluorescent Hypoxia Sensors
Tabea Marie Fleischhammer, Sandra Dienemann, Nico Ulber, Iliyana Pepelanova, and Antonina Lavrentieva

3.     Mapping the Fate of Hypoxic Cells using an Irreversible Fluorescent Switch
Alyssa G. Weinstein, Daniele M. Gilkes, and Inês Godet

4.     Application of a Specific and Sensitive NQO1 Turn-On Near-Infrared Fluorescence Probe for Live Cancer Cell and Xenografted Tumor Imaging in Nude Mice
Kalkunte S. Srivenugopal, Viswanath Arutla, Surendra R. Punganuru, and AEM Adnan Khan

Part II Non-Fluorescent Reporter Methods for Hypoxia Detection

5.     A Luciferase Reporter Assay to Detect Cellular Hypoxia In Vitro
Masayuki Inubushi, Yasuto Takeuchi, Chika Murai, and Yoshimasa Kitagawa

6.     Oxygen Assessment in Tumors In Vivo using Phosphorescence Lifetime Imaging Microscopy
Anastasia D. Komarova, Vladislav I. Shcheslavskiy, Anton A. Plekhanov, Marina A. Sirotkina, Leonid N. Bochkarev, and Marina V. Shirmanova

7.     Radionuclide Reporter Imaging to Visualize Tumor Hypoxia Ex Vivo and In Vivo
Masayuki Inubushi, Yasuto Takeuchi, and Yoshimasa Kitagawa

8.     Measuring Pericellular Oxygen Tension for In Vitro Cell Culture
Zachary J. Rogers, Khushbu Bhatt, and Sidi A. Bencherif

9.     A Review of Hypoxia Imaging Using 18F-Fluoromisonidazole Positron Emission Tomography
Kenji Hirata, Shiro Watanabe, Yoshimasa Kitagawa, and Kohsuke Kudo

10. Photoacoustic Lifetime Imaging of Hypoxia
Qi Shao and Joshua G. Punnoose

11. Staining Hypoxic Areas in Frozen and FFPE Tissue Sections with Hypoxyprobe™
Harsh H. Oza, Esther Ng, and Daniele M. Gilkes

Part III Methods to Detect Hypoxia Inducible Factors and their Downstream Targets

12. Multiplex Immunofluorescence Staining Protocol for Dual Imaging of Hypoxia-Inducible Factors 1 and 2 on Formalin-Fixed Paraffin-Embedded Samples
Harsh H. Oza and Daniele M. Gilkes

13. Detecting Hypoxia-Inducible Factor Levels and Activity
Maimon E. Hubbi

Part IV Methods to Detect Hypoxia-Induced Changes in Cell Metabolism

14. Metabolomic Investigations into Hypoxia-Mediated Metabolic Reprogramming of Pancreatic Cancer Cells
Vira Chumak, Girish H. Rajacharya, and Pankaj K. Singh

15. Measurement of Metabolic Alteration in Immune Cells under Hypoxia
Yuki Fujii and Kamiya Mehla

Part V Methods to Simulate Hypoxia, Evaluate Oxygen Consumption Rates, and Measure FIH Activity Levels

16. Evaluation of Oxygen Consumption Rates In Situ
Anqi Li, Yuan Qin, Ying Zhang, Xiaoqun Zhen, and Guohua Gong

17. Cleanroom-Free Microfluidic Device for Natural Induction of Hypoxia in 2-D and 3-D Tumor Models
Jeong Min Oh and Keyue Shen

18. Oxomer- and Reporter Gene-Based Analysis of FIH Activity in Cells
Yulia L. Volkova, Agnieszka E. Jucht, and Carsten C. Scholz

Subject Index List…

Erscheinungsdatum	08.02.2024
Reihe/Serie	Methods in Molecular Biology ; 2755
Zusatzinfo	58 Tables, color; 59 Illustrations, color; 10 Illustrations, black and white; XI, 266 p. 69 illus., 59 illus. in color.
Verlagsort	New York
Sprache	englisch
Maße	178 x 254 mm
Gewicht	792 g
Einbandart	gebunden
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
Themenwelt	Medizin / Pharmazie ► Studium
Schlagworte	18F-Fluoromisonidazole Positron Emission Tomography • blood oxygenation level dependent (BOLD) • electrochemical oxygen sensors • fluorescent reporters • Photoacoustic Imaging
ISBN-10	1-0716-3632-4 / 1071636324
ISBN-13	978-1-0716-3632-9 / 9781071636329
Zustand	Neuware