Plant-Virus Interactions

Elizabeth P.B. Fontes, Kristiina Mäkinen (Herausgeber)

Buch | Hardcover

316 Seiten

2023 | 1st ed. 2024
Springer-Verlag New York Inc.
978-1-0716-3484-4 (ISBN)

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This detailed volume provides practical guidance on techniques in plant-virus interaction research, from targeting specific molecular interactions within the virus-host interactome to the identification of the complete virus-host protein-protein interaction network. After chapters on acquiring the necessary molecular tools, the book continues with biochemical and genetic approaches to confirming protein-protein interactions both in vivo and in vitro, procedures and protocols for assessing replication, translation, viral genome movement, and insect transmission, as well as techniques for detecting multiple molecular interactions between the host and the virus and monitoring immune hubs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Plant-Virus Interactions serves as a valuable resource for understanding the protein-protein interaction network between the virus and the host, crucial for comprehending the life cycle of a virus and for developing strategies for broad-spectrum and long-lasting resistance against viral infections.

lt;p>1. Computational Pipeline for Detection of Plant RNA Viruses Using High-Throughput Sequencing

Livia Donaire and Miguel A. Aranda

2. Taxonomic Classification of Geminiviruses Based on Pairwise Sequence Comparisons

João Paulo Herrera da Silva and F. Murilo Zerbini

3. Initiation of Plant Virus Infections by Agroinfiltration of Infectious Viral cDNAs (icDNAs)

Maija Pollari

4. Construction of Geminivirus Infectious Clones for Agroinoculation into Plants

Ivair J. Morais, Alice K. Inoue-Nagata, and Erich Y.T. Nakasu

5. Agroinoculation of Geminiviral Infectious Clones into Plants

Ivair J. Morais, Yanca F. M. Ferreira, Erich Y.T. Nakasu, Tatsuya Nagata, and Alice K. Inoue-Nagata

6. Begomovirus Inoculation in Arabidopsis and Cassava

José T. Ascencio-Ibáñez, Mary M. Dallas, and Linda Hanley-Bowdoin

7. Absolute and Relative Quantification of Single-Stranded Positive Sense RNA Virus from Plant Tissue

Pinky Dutta and Kristiina Mäkinen

8. Quantification of Virion-Sense and Complementary-Sense DNA Strands of Circular Single-Stranded DNA Viruses

Edgar A. Rodríguez-Negrete and Ana Grande-Pérez

9. Replication Assay of Begomovirus in Arabidopsis Protoplasts

Laura Gonçalves Costa Martins and Elizabeth P.B. Fontes

10. Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye

Yinzi Li and Aiming Wang

11. Ribosome Profiling of Plants

Jahanara Sonia, Pulkit Kanodia, Zachary Lozier, and W. Allen Miller

12. Experimental Transmission of Plant Viruses by Aphids or Whiteflies

Irene Ontiveros, Juan Antonio Diaz-Pendón, and Juan José López-Moya

13. Quantification of Plant Virus Seed Transmission Rate in Arabidopsis thaliana

Miriam Gil-Valle, Cristina Sáez, Nuria Montes, and Israel Pagán

14. Protein-Protein Interaction via Two-Hybrid Assay in Yeast

Christiane Eliza Motta Duarte and Nivea Costa Euclydes

15. Bimolecular Fluorescence Complementation (BIFC) in Host-Virus Interactions

Fredy Davi Albuquerque Silva, João Paulo Batista Machado, and Pedro Augusto Braga dos Reis

16. Co-Immunoprecipitation Assays to Detect Protein-Protein Interactions

Mengling Huang, Xiao Yu, and Bo Li

17. Split-Luciferase Complementation Imaging Assay in Virus-Plant Interactions

Huang Tan, Xi Zhang, and Rosa Lozano-Duran

18. The Split-Luciferase Complementation Assay to Detect and Quantify Protein-Protein Interactions In Planta

Eduardo Bassi Simoni and Célio Cabral Oliveira

19. TurboID-Based Proximity Labeling: A Method to Decipher Protein-Protein Interactions in Plants

Yuanyuan Li, Yongliang Zhang, and Savithramma P. Dinesh-Kumar

20. Virus-Induced Heritable Gene Editing in Plants

Ugrappa Nagalakshmi, Nathan Meier, and Savithramma P. Dinesh-Kumar

21. Immunoprecipitation Followed by Mass Spectrometry: An Approach for Identifying Host-Viral Protein-Protein Interactions

Laura Medina-Puche and Rosa Lozano-Duran

22. In Situ Protein Microarray for Identifying the Geminivirus-Arabidopsis Interactome

Junshi Yazaki and Maximiller Dal-Bianco

Erscheinungsdatum	23.11.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	47 Illustrations, color; 15 Illustrations, black and white; XI, 316 p. 62 illus., 47 illus. in color. With online files/update.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Naturwissenschaften ► Biologie ► Botanik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	DNA and RNA virus inoculation • Immune hubs • plant viruses • protein-protein interactions • Viral genome cloning • Viral molecular interactions • Virus-host interactome
ISBN-10	1-0716-3484-4 / 1071634844
ISBN-13	978-1-0716-3484-4 / 9781071634844
Zustand	Neuware