Tissue-Resident Macrophages
Springer-Verlag New York Inc.
978-1-0716-3436-3 (ISBN)
Thorough and practical, Tissue-Resident Macrophages: Methods and Protocols provides scientists entering the macrophage field with information and tools that allow them to dive into the state-of-the-art methodology used in this vital field.
lt;p>1. Macrophage Development and Function
Nikola Makdissi
2. Fate Mapping Macrophages: From Ontogeny to Functions
Anna Ahlback and Rebecca Gentek
3. Studying Autophagy in Microglia: Overcoming the Obstacles
Ainhoa Plaza-Zabala and Amanda Sierra
4. Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-Seq
Fabian Hersperger, Melanie Kastl, Katrin Paeschke, and Katrin Kierdorf
5. Isolation of Tissue Macrophages in Adult Zebrafish
Mireia Rovira, Jennifer Pozo, Magali Miserocchi, and Valérie Wittamer
6. Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ
Stephen Huang, Dylan Carter-Cusack, Emma Maxwell, Omkar L. Patkar, Katharine M. Irvine, and David A. Hume
7. Phenotyping of Macrophages in Human Immune System Mice
Leonie Voss, Carmen Reitinger, and Anja Lux
8. Fate-Mapping of Yolk Sac-Derived Macrophages
Iva Splichalova and Elvira Mass
9. Fate-Mapping of Hematopoietic Stem Cell-Derived Macrophages
Katharina Mauel and Elvira Mass
10. Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue
Dailia Ribeiro, Seniz Yüksel, Andreas Dolf, and Dagmar Wachten
11. Isolation and Flow Cytometry Analysis of Macrophages from the Dermis
Aaron James Forde and Julia Kolter
12. Isolation and Flow Cytometry Analysis of Macrophages from the Kidney
Sarah J. Bland, Alex Yashchenko, and Kurt A. Zimmerman
13. Isolation and Flow Cytometry Analysis of Intestinal Macrophages
Maria Francesca Viola and Guy Boeckxstaens
14. Isolation and Characterization of Testis Macrophages Using Flow Cytometry
Myriam Sekias, Myriam Baratin, and Marc Bajenoff
15. Studying Macrophages in the Murine Fatty Liver Using Flow Cytometry and Confocal Microscopy
Zhuangzhuang Liu, Pieter A. Louwe, and Charlotte L. Scott
16. Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages
Clara Jana-Lui Busch, Sethuraman Subramanian, Javier Linares, Jérémy Favret, Ridzky Anis Advent Yuda, and Michael H. Sieweke
17. Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach
Jung-Seok Kim, Zhana Haimon, Sigalit Boura-Halfon, and Steffen Jung
18. Spectral Flow Cytometry Analysis of Resident Tissue Macrophages
Wan Ting Kong, Mathilde Bied, and Florent Ginhoux
19. Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging
David Alejandro Bejarano and Andreas Schlitzer
20. 3D Imaging of Macrophages in Complete Organs
Carole Siret and Serge A. van de Pavert
21. Whole-Mount Imaging of Adipose Tissue Macrophages
Lydia Sorokin and Luis Henrique Corrêa
22. Functional In Vivo Imaging of Macrophages
Anja Wegner, Ralph Palmisano, and Stefan Uderhardt
23. Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy
Karolin W. Hublitz and Efstathios G. Stamatiades
24. Combined Host-Pathogen Fate Mapping to Investigate Lung Macrophages in Viral Infection
Sebastian Baasch, Julia Henschel, and Philipp Henneke
25. Measuring the Metabolic State of Tissue-Resident Macrophages via SCENITH
Andrea Vogel, Paulina Garcia Gonzalez, and Rafael J. Argüello
26. Analyzing Fcg-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA)
Sibel Kara and Falk Nimmerjahn
27. Studying Efferocytosis Dynamics in Tissue-Resident Macrophages Ex Vivo
Irene Aranda-Pardos, Achmet Imam-Chasan, and Noelia Alonso-Gonzalez
28. Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparation and Inflammasome Stimulation
Marta Lovotti, Matthew S.J. Mangan, Róisín M. McManus, Kateryna Shkarina, Matilde B. Vasconcelos, and Eicke Latz
29. Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation
Marta Lovotti, Matthew S.J. Mangan, Róisín M. McManus, Kateryna Shkarina, Matilde B. Vasconcelos, and Eicke Latz
30. Detection of G-Quadruplex DNA Structures in Macrophages
Melanie Kastl, Fabian Hersperger, Katrin Kierdorf, and Katrin Paeschke
31. Adaptation of Human iPSC-Derived Macrophages Towards an Alveolar Macrophage-Like Phenotype Post Intra-Pulmonary Transfer into Murine Models
Miriam Hetzel, Ingrid Gensch, Mania Ackermann, and Nico Lachmann
32. Tackling Tissue Macrophage Heterogeneity by SplitCre Transgenesis
Sigalit Boura-Halfon, Rebecca Haffner-Krausz, Shifra Ben-Dor, Jung-Seok Kim, and Steffen Jung
33. Automated Cell Counting of Macrophages In Situ
Ouzéna Bouadi and Tuan Leng Tay
34. Morphometric Analyses of Macrophages
Jan N. Hansen
35. Combined Analysis of mRNA Expression and Open Chromatin in Microglia
Rebekka Scholz, Desirée Brösamle, Xidi Yuan, Jonas J. Neher, and Marc Beyer
| Erscheinungsdatum | 01.09.2023 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology |
| Zusatzinfo | 120 Illustrations, color; 8 Illustrations, black and white; XVI, 576 p. 128 illus., 120 illus. in color. With online files/update. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Studium ► Querschnittsbereiche ► Infektiologie / Immunologie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | Efferocytosis • Inflammasome activation • Innate immune system • Model organisms • Monocyte-derived macrophages • phagocytes |
| ISBN-10 | 1-0716-3436-4 / 1071634364 |
| ISBN-13 | 978-1-0716-3436-3 / 9781071634363 |
| Zustand | Neuware |
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