Forensic DNA Typing Protocols

Buch | Hardcover

280 Seiten

2004
Humana Press Inc. (Verlag)
978-1-58829-264-3 (ISBN)

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A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics.

A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

Forensic DNA-Typing Technologies.- DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR.- A Real-Time PCR Protocol to Determine the Number of Amelogenin (X-Y) Gene Copies From Forensic DNA Samples.- Species Determination.- Constructing STR Multiplex Assays.- Y Chromosome STR Typing.- Using Online Databases for Developing SNP Markers of Forensic Interest.- SNP Typing in Forensic Genetics.- Genotyping SNPs With the LightCycler.- Universal Tag Arrays in Forensic SNP Analysis.- SNP Typing on the NanoChip Electronic Microarray.- Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS.- Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing.- SNaPshot Typing of Mitochondrial DNA Coding Region Variants.- Typing of Y Chromosome SNPs With Multiplex PCR Methods.- Y Chromosome SNP Analysis Using the Single-Base Extension.- SNaPshot for Pharmacogenetics by Minisequencing.- Methods for the Study of Ancient DNA.- Protocols for Ancient DNA Typing.

Erscheint lt. Verlag	30.11.2004
Reihe/Serie	Methods in Molecular Biology ; 297
Zusatzinfo	81 Illustrations, black and white; X, 280 p. 81 illus.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 2. Studienabschnitt (Klinik) ► Rechtsmedizin
	Studium ► Querschnittsbereiche ► Prävention / Gesundheitsförderung
ISBN-10	1-58829-264-9 / 1588292649
ISBN-13	978-1-58829-264-3 / 9781588292643
Zustand	Neuware